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Using Bcr-Abl to examine mechanisms by which abl kinase regulates morphogenesis in Drosophila.

Publication ,  Journal Article
Stevens, TL; Rogers, EM; Koontz, LM; Fox, DT; Homem, CCF; Nowotarski, SH; Artabazon, NB; Peifer, M
Published in: Mol Biol Cell
January 2008

Signaling by the nonreceptor tyrosine kinase Abelson (Abl) plays key roles in normal development, whereas its inappropriate activation helps trigger the development of several forms of leukemia. Abl is best known for its roles in axon guidance, but Abl and its relatives also help regulate embryonic morphogenesis in epithelial tissues. Here, we explore the role of regulation of Abl kinase activity during development. We first compare the subcellular localization of Abl protein and of active Abl, by using a phosphospecific antibody, providing a catalog of places where Abl is activated. Next, we explore the consequences for morphogenesis of overexpressing wild-type Abl or expressing the activated form found in leukemia, Bcr-Abl. We find dose-dependent effects of elevating Abl activity on morphogenetic movements such as head involution and dorsal closure, on cell shape changes, on cell protrusive behavior, and on the organization of the actin cytoskeleton. Most of the effects of Abl activation parallel those caused by reduction in function of its target Enabled. Abl activation leads to changes in Enabled phosphorylation and localization, suggesting a mechanism of action. These data provide new insight into how regulated Abl activity helps direct normal development and into possible biological functions of Bcr-Abl.

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Published In

Mol Biol Cell

DOI

EISSN

1939-4586

Publication Date

January 2008

Volume

19

Issue

1

Start / End Page

378 / 393

Location

United States

Related Subject Headings

  • rho GTP-Binding Proteins
  • Pseudopodia
  • Protein-Tyrosine Kinases
  • Protein Transport
  • Phosphorylation
  • Morphogenesis
  • Male
  • Fusion Proteins, bcr-abl
  • Female
  • Enzyme Activation
 

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Stevens, T. L., Rogers, E. M., Koontz, L. M., Fox, D. T., Homem, C. C. F., Nowotarski, S. H., … Peifer, M. (2008). Using Bcr-Abl to examine mechanisms by which abl kinase regulates morphogenesis in Drosophila. Mol Biol Cell, 19(1), 378–393. https://doi.org/10.1091/mbc.e07-01-0008
Stevens, Traci L., Edward M. Rogers, Laura M. Koontz, Donald T. Fox, Catarina C. F. Homem, Stephanie H. Nowotarski, Nicholas B. Artabazon, and Mark Peifer. “Using Bcr-Abl to examine mechanisms by which abl kinase regulates morphogenesis in Drosophila.Mol Biol Cell 19, no. 1 (January 2008): 378–93. https://doi.org/10.1091/mbc.e07-01-0008.
Stevens TL, Rogers EM, Koontz LM, Fox DT, Homem CCF, Nowotarski SH, et al. Using Bcr-Abl to examine mechanisms by which abl kinase regulates morphogenesis in Drosophila. Mol Biol Cell. 2008 Jan;19(1):378–93.
Stevens, Traci L., et al. “Using Bcr-Abl to examine mechanisms by which abl kinase regulates morphogenesis in Drosophila.Mol Biol Cell, vol. 19, no. 1, Jan. 2008, pp. 378–93. Pubmed, doi:10.1091/mbc.e07-01-0008.
Stevens TL, Rogers EM, Koontz LM, Fox DT, Homem CCF, Nowotarski SH, Artabazon NB, Peifer M. Using Bcr-Abl to examine mechanisms by which abl kinase regulates morphogenesis in Drosophila. Mol Biol Cell. 2008 Jan;19(1):378–393.

Published In

Mol Biol Cell

DOI

EISSN

1939-4586

Publication Date

January 2008

Volume

19

Issue

1

Start / End Page

378 / 393

Location

United States

Related Subject Headings

  • rho GTP-Binding Proteins
  • Pseudopodia
  • Protein-Tyrosine Kinases
  • Protein Transport
  • Phosphorylation
  • Morphogenesis
  • Male
  • Fusion Proteins, bcr-abl
  • Female
  • Enzyme Activation