Identification of cells of skin lympho monocyte infiltrates in patients with urticaria
The differentiation between B cells (bone marrow derived lymphocytes), T cells (thymus derived lymphocytes) and histiocytes in tissue sections through utilization of known differences in cell membrane receptors was studied. Sheep erythrocytes (E) were coated with either IgG or IgM antibody (A) to form IgGEA or IgMEA. IgMEA was incubated with mouse complement (C) to form IgMEAC. Cryosections were layered with IgMEAC, IgMEA or IgGEA and subpopulations of mononuclear cells could be distinguished from one another by the adherence or non adherence of these reagents. B cells bound IgMEAC, but not IgMEA or IgGEA; histiocytes bound IgMEAC and IgGEA but not IgMEA and T cells failed to bind any of these reagents. Controls were performed on normal human spleen and lymph nodes. In 8 patients with 'chronic urticaria' of unidentifiable cause and without underlying disease, the mononuclear cells of skin infiltrates were identified, by exclusion, as T cells, binding neither IgMEAC nor IgGEA.
Gerna Torsellini, M; Edelson, RL; Frank, MM; Kaplan, A
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