Detection of diverse variants of human immunodeficiency virus-1 groups M, N, and O and simian immunodeficiency viruses from chimpanzees by using generic pol and env primer pairs.


Journal Article

Human immunodeficiency virus type 1 (HIV-1) infection of humans is the result of independent cross-species transmissions of simian immunodeficiency viruses (SIVcpz) from naturally infected chimpanzees (Pan troglodytes troglodytes) to man. To develop a polymerase chain reaction-based assay capable of detecting members of all major phylogenetic SIVcpz and HIV-1 lineages (groups M, N, and O), primer pairs in conserved pol and env regions were designed. Both primer sets amplified 99%) in amplifying viral sequences from plasma taken from patients infected with HIV-1 group M (n=226) and O (n=17) viruses.

Full Text

Duke Authors

Cited Authors

  • Yang, C; Dash, BC; Simon, F; van der Groen, G; Pieniazek, D; Gao, F; Hahn, BH; Lal, RB

Published Date

  • May 2000

Published In

Volume / Issue

  • 181 / 5

Start / End Page

  • 1791 - 1795

PubMed ID

  • 10823786

Pubmed Central ID

  • 10823786

International Standard Serial Number (ISSN)

  • 0022-1899

Digital Object Identifier (DOI)

  • 10.1086/315439


  • eng

Conference Location

  • United States