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Evolution and probable transmission of intersubtype recombinant human immunodeficiency virus type 1 in a Zambian couple.

Publication ,  Journal Article
Salminen, MO; Carr, JK; Robertson, DL; Hegerich, P; Gotte, D; Koch, C; Sanders-Buell, E; Gao, F; Sharp, PM; Hahn, BH; Burke, DS; McCutchan, FE
Published in: J Virol
April 1997

The extraordinary genetic diversity of human immunodeficiency virus type 1 (HIV-1) results from the introduction of mutations by an error-prone reverse transcriptase and from recombination of the two RNA genomes packaged in the virion during the synthesis of proviral DNA. The occurrence of multiple, genetically distant HIV-1 subtypes and their geographic intermixing set up conditions for dramatic, rather than gradual, changes in genotype whenever genomes from different subtypes are copackaged in virions. Here we describe, for the first time, the sequential generation of multiple different, but related, intersubtype HIV-1 recombinants within an infected individual. Full-length gag and env genes were recovered directly from peripheral blood mononuclear cells or from primary virus cultures, using serial blood samples from a Zambian woman and a sample from her spouse. DNA sequencing and phylogenetic analysis established that two different A/C recombinant forms of HIV-1 predominated at two time points in the woman. A related but distinct recombinant HIV-1 was recovered from her spouse. Intersubtype recombination apparently played a central role in the evolution of HIV-1 in this couple and may contribute substantially to the rapid emergence of HIV-1 variants whenever mixed-subtype HIV-1 infections occur.

Duke Scholars

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

April 1997

Volume

71

Issue

4

Start / End Page

2647 / 2655

Location

United States

Related Subject Headings

  • Zambia
  • Virology
  • Recombination, Genetic
  • Phylogeny
  • Molecular Sequence Data
  • Male
  • Humans
  • HIV-1
  • HIV Seropositivity
  • Female
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Salminen, M. O., Carr, J. K., Robertson, D. L., Hegerich, P., Gotte, D., Koch, C., … McCutchan, F. E. (1997). Evolution and probable transmission of intersubtype recombinant human immunodeficiency virus type 1 in a Zambian couple. J Virol, 71(4), 2647–2655. https://doi.org/10.1128/JVI.71.4.2647-2655.1997
Salminen, M. O., J. K. Carr, D. L. Robertson, P. Hegerich, D. Gotte, C. Koch, E. Sanders-Buell, et al. “Evolution and probable transmission of intersubtype recombinant human immunodeficiency virus type 1 in a Zambian couple.J Virol 71, no. 4 (April 1997): 2647–55. https://doi.org/10.1128/JVI.71.4.2647-2655.1997.
Salminen MO, Carr JK, Robertson DL, Hegerich P, Gotte D, Koch C, et al. Evolution and probable transmission of intersubtype recombinant human immunodeficiency virus type 1 in a Zambian couple. J Virol. 1997 Apr;71(4):2647–55.
Salminen, M. O., et al. “Evolution and probable transmission of intersubtype recombinant human immunodeficiency virus type 1 in a Zambian couple.J Virol, vol. 71, no. 4, Apr. 1997, pp. 2647–55. Pubmed, doi:10.1128/JVI.71.4.2647-2655.1997.
Salminen MO, Carr JK, Robertson DL, Hegerich P, Gotte D, Koch C, Sanders-Buell E, Gao F, Sharp PM, Hahn BH, Burke DS, McCutchan FE. Evolution and probable transmission of intersubtype recombinant human immunodeficiency virus type 1 in a Zambian couple. J Virol. 1997 Apr;71(4):2647–2655.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

April 1997

Volume

71

Issue

4

Start / End Page

2647 / 2655

Location

United States

Related Subject Headings

  • Zambia
  • Virology
  • Recombination, Genetic
  • Phylogeny
  • Molecular Sequence Data
  • Male
  • Humans
  • HIV-1
  • HIV Seropositivity
  • Female