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Enrichment of peripheral blood stem cells in a primate model following administration of a single dose of rh-IL-1 beta.

Publication ,  Journal Article
Gasparetto, C; Smith, C; Gillio, A; Stoppa, AM; Moore, MA; O'Reilly, RJ
Published in: Bone Marrow Transplant
November 1994

In a pre-clinical primate model, the effect of a single intravenous injection of rh-GM-CSF (50 micrograms/kg), rh-IL3 (20 micrograms/kg), rh-IL-1 beta (1 micrograms/kg), rh-G-CSF (50 micrograms/kg) and rh-pIXY 321 (50 micrograms/kg) on peripheral blood mononuclear cell and hematopoietic stem cell concentration was determined. The results indicated that administration of a single dose of rh-IL-1 beta increased the concentrations of hematopoietic stem cells, including CFU-GM progenitors and precursors to CFU-GM. No toxicity was noted with this procedure. None of the remaining cytokines tested caused a significant increase in the peripheral blood hematopoietic stem cell concentration when administered in this manner and only G-CSF caused an increase in peripheral blood mononuclear cell concentrations. Autologous transplantation with limiting numbers of peripheral blood mononuclear cells (1 x 10(7) cells/kg) collected either 24 or 72 h following injection of 1 microgram/kg rh-IL-1 beta conferred a survival advantage to recipients compared with controls. These results imply that a single intravenous administration of rh-IL-1 beta may increase the concentration of peripheral blood stem cells to levels sufficient for transplantation.

Duke Scholars

Published In

Bone Marrow Transplant

ISSN

0268-3369

Publication Date

November 1994

Volume

14

Issue

5

Start / End Page

717 / 723

Location

England

Related Subject Headings

  • Transplantation, Autologous
  • Recombinant Proteins
  • Recombinant Fusion Proteins
  • Neutrophils
  • Models, Biological
  • Male
  • Macaca fascicularis
  • Leukocyte Count
  • Interleukin-3
  • Interleukin-1
 

Citation

APA
Chicago
ICMJE
MLA
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Gasparetto, C., Smith, C., Gillio, A., Stoppa, A. M., Moore, M. A., & O’Reilly, R. J. (1994). Enrichment of peripheral blood stem cells in a primate model following administration of a single dose of rh-IL-1 beta. Bone Marrow Transplant, 14(5), 717–723.
Gasparetto, C., C. Smith, A. Gillio, A. M. Stoppa, M. A. Moore, and R. J. O’Reilly. “Enrichment of peripheral blood stem cells in a primate model following administration of a single dose of rh-IL-1 beta.Bone Marrow Transplant 14, no. 5 (November 1994): 717–23.
Gasparetto C, Smith C, Gillio A, Stoppa AM, Moore MA, O’Reilly RJ. Enrichment of peripheral blood stem cells in a primate model following administration of a single dose of rh-IL-1 beta. Bone Marrow Transplant. 1994 Nov;14(5):717–23.
Gasparetto, C., et al. “Enrichment of peripheral blood stem cells in a primate model following administration of a single dose of rh-IL-1 beta.Bone Marrow Transplant, vol. 14, no. 5, Nov. 1994, pp. 717–23.
Gasparetto C, Smith C, Gillio A, Stoppa AM, Moore MA, O’Reilly RJ. Enrichment of peripheral blood stem cells in a primate model following administration of a single dose of rh-IL-1 beta. Bone Marrow Transplant. 1994 Nov;14(5):717–723.

Published In

Bone Marrow Transplant

ISSN

0268-3369

Publication Date

November 1994

Volume

14

Issue

5

Start / End Page

717 / 723

Location

England

Related Subject Headings

  • Transplantation, Autologous
  • Recombinant Proteins
  • Recombinant Fusion Proteins
  • Neutrophils
  • Models, Biological
  • Male
  • Macaca fascicularis
  • Leukocyte Count
  • Interleukin-3
  • Interleukin-1