Phage display for target-based antibacterial drug discovery.

Journal Article (Journal Article)

Increasing bacterial drug resistance and hard-to-eradicate opportunistic infections have created a need for new antibiotics. Sequencing of microbial genomes has yielded many new potential targets for antibacterial drug discovery. However, little is known about the biochemical activities of many of these targets, making it difficult to develop HTS assays for them. Peptides isolated by phage display can be used as 'surrogate ligands' in competition assays for screening of targets of unknown function with small-molecule libraries. These screening assays can be adapted into a variety of high-throughput formats, including those based on radioactive, luminescence or fluorescence detection.

Full Text

Duke Authors

Cited Authors

  • Christensen, DJ; Gottlin, EB; Benson, RE; Hamilton, PT

Published Date

  • July 1, 2001

Published In

Volume / Issue

  • 6 / 14

Start / End Page

  • 721 - 727

PubMed ID

  • 11445463

Electronic International Standard Serial Number (EISSN)

  • 1878-5832

Digital Object Identifier (DOI)

  • 10.1016/s1359-6446(01)01853-0


  • eng

Conference Location

  • England