Crosstalk between bacterial chemotaxis signal transduction proteins and regulators of transcription of the Ntr regulon: evidence that nitrogen assimilation and chemotaxis are controlled by a common phosphotransfer mechanism.


Journal Article

We demonstrate by using purified bacterial components that the protein kinases that regulate chemotaxis and transcription of nitrogen-regulated genes, CheA and NRII, respectively, have cross-specificities: CheA can phosphorylate the Ntr transcription factor NRI and thereby activate transcription from the nitrogen-regulated glnA promoter, and NRII can phosphorylate CheY. In addition, we find that a high intracellular concentration of a highly active mutant form of NRII can suppress the smooth-swimming phenotype of a cheA mutant. These results argue strongly that sensory transduction in the Ntr and Che systems involves a common protein phosphotransfer mechanism.

Full Text

Duke Authors

Cited Authors

  • Ninfa, AJ; Ninfa, EG; Lupas, AN; Stock, A; Magasanik, B; Stock, J

Published Date

  • August 1988

Published In

Volume / Issue

  • 85 / 15

Start / End Page

  • 5492 - 5496

PubMed ID

  • 3041412

Pubmed Central ID

  • 3041412

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.85.15.5492


  • eng

Conference Location

  • United States