Crosstalk between bacterial chemotaxis signal transduction proteins and regulators of transcription of the Ntr regulon: evidence that nitrogen assimilation and chemotaxis are controlled by a common phosphotransfer mechanism.
Published
Journal Article
We demonstrate by using purified bacterial components that the protein kinases that regulate chemotaxis and transcription of nitrogen-regulated genes, CheA and NRII, respectively, have cross-specificities: CheA can phosphorylate the Ntr transcription factor NRI and thereby activate transcription from the nitrogen-regulated glnA promoter, and NRII can phosphorylate CheY. In addition, we find that a high intracellular concentration of a highly active mutant form of NRII can suppress the smooth-swimming phenotype of a cheA mutant. These results argue strongly that sensory transduction in the Ntr and Che systems involves a common protein phosphotransfer mechanism.
Full Text
Duke Authors
Cited Authors
- Ninfa, AJ; Ninfa, EG; Lupas, AN; Stock, A; Magasanik, B; Stock, J
Published Date
- August 1988
Published In
Volume / Issue
- 85 / 15
Start / End Page
- 5492 - 5496
PubMed ID
- 3041412
Pubmed Central ID
- 3041412
International Standard Serial Number (ISSN)
- 0027-8424
Digital Object Identifier (DOI)
- 10.1073/pnas.85.15.5492
Language
- eng
Conference Location
- United States