A new model of the blood--brain barrier: co-culture of neuronal, endothelial and glial cells under dynamic conditions.

Journal Article (Journal Article)

Developing in vitro blood-brain barrier (BBB) models that closely mimic the natural state is important for theoretical and practical applications, including drug development. We previously developed an in vitro BBB model based on co-culturing endothelial cells with glia in the presence of flow on hollow fiber tube culture substrates. We now report that this dynamic in vitro BBB (DIV-BBB) can be successfully used to co-culture differentiated serotonergic neurons in the presence of a BBB. These neurons demonstrated fluoxetine-sensitive serotonin (5HT) uptake and depolarization-induced release of [3H]5HT. Our results demonstrate that the DIV-BBB is a suitable model for culturing of neurons in a quasi-physiological microenvironment and in the presence of a high-resistance, stereoselective BBB.

Full Text

Duke Authors

Cited Authors

  • Stanness, KA; Neumaier, JF; Sexton, TJ; Grant, GA; Emmi, A; Maris, DO; Janigro, D

Published Date

  • December 16, 1999

Published In

Volume / Issue

  • 10 / 18

Start / End Page

  • 3725 - 3731

PubMed ID

  • 10716199

International Standard Serial Number (ISSN)

  • 0959-4965

Digital Object Identifier (DOI)

  • 10.1097/00001756-199912160-00001


  • eng

Conference Location

  • England