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Phosphorylation of RNA polymerase II C-terminal domain and transcriptional elongation.

Publication ,  Journal Article
O'Brien, T; Hardin, S; Greenleaf, A; Lis, JT
Published in: Nature
July 7, 1994

The carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II is essential in vivo, and is found in either an unphosphorylated (IIa) or hyperphosphorylated (IIo) form. The Drosophila uninduced hsp70 and hsp26 genes, and the constitutively expressed beta-1 tubulin and Gapdh-2 genes, contain an RNA polymerase II complex which pauses after synthesizing a short transcript. We report here that, using an in vivo ultraviolet crosslinking technique and antibodies directed against the IIa and IIo forms of the CTD, these paused polymerases have an unphosphorylated CTD. For genes containing a 5' paused polymerase, passage of the paused RNA polymerase into an elongationally competent mode in vivo coincides with phosphorylation of the CTD. Also, the level of phosphorylation of the CTD of elongating polymerases is shown not to be related to the level of transcription, but is promoter specific.

Duke Scholars

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

July 7, 1994

Volume

370

Issue

6484

Start / End Page

75 / 77

Location

England

Related Subject Headings

  • Ultraviolet Rays
  • Tubulin
  • Transcription, Genetic
  • Restriction Mapping
  • RNA Polymerase II
  • Phosphorylation
  • Heat-Shock Proteins
  • General Science & Technology
  • Drosophila
  • DNA
 

Citation

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MLA
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O’Brien, T., Hardin, S., Greenleaf, A., & Lis, J. T. (1994). Phosphorylation of RNA polymerase II C-terminal domain and transcriptional elongation. Nature, 370(6484), 75–77. https://doi.org/10.1038/370075a0
O’Brien, T., S. Hardin, A. Greenleaf, and J. T. Lis. “Phosphorylation of RNA polymerase II C-terminal domain and transcriptional elongation.Nature 370, no. 6484 (July 7, 1994): 75–77. https://doi.org/10.1038/370075a0.
O’Brien T, Hardin S, Greenleaf A, Lis JT. Phosphorylation of RNA polymerase II C-terminal domain and transcriptional elongation. Nature. 1994 Jul 7;370(6484):75–7.
O’Brien, T., et al. “Phosphorylation of RNA polymerase II C-terminal domain and transcriptional elongation.Nature, vol. 370, no. 6484, July 1994, pp. 75–77. Pubmed, doi:10.1038/370075a0.
O’Brien T, Hardin S, Greenleaf A, Lis JT. Phosphorylation of RNA polymerase II C-terminal domain and transcriptional elongation. Nature. 1994 Jul 7;370(6484):75–77.
Journal cover image

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

July 7, 1994

Volume

370

Issue

6484

Start / End Page

75 / 77

Location

England

Related Subject Headings

  • Ultraviolet Rays
  • Tubulin
  • Transcription, Genetic
  • Restriction Mapping
  • RNA Polymerase II
  • Phosphorylation
  • Heat-Shock Proteins
  • General Science & Technology
  • Drosophila
  • DNA