Phosphorylation of RNA polymerase II C-terminal domain and transcriptional elongation.
Published
Journal Article
The carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II is essential in vivo, and is found in either an unphosphorylated (IIa) or hyperphosphorylated (IIo) form. The Drosophila uninduced hsp70 and hsp26 genes, and the constitutively expressed beta-1 tubulin and Gapdh-2 genes, contain an RNA polymerase II complex which pauses after synthesizing a short transcript. We report here that, using an in vivo ultraviolet crosslinking technique and antibodies directed against the IIa and IIo forms of the CTD, these paused polymerases have an unphosphorylated CTD. For genes containing a 5' paused polymerase, passage of the paused RNA polymerase into an elongationally competent mode in vivo coincides with phosphorylation of the CTD. Also, the level of phosphorylation of the CTD of elongating polymerases is shown not to be related to the level of transcription, but is promoter specific.
Full Text
Duke Authors
Cited Authors
- O'Brien, T; Hardin, S; Greenleaf, A; Lis, JT
Published Date
- July 7, 1994
Published In
Volume / Issue
- 370 / 6484
Start / End Page
- 75 - 77
PubMed ID
- 8015613
Pubmed Central ID
- 8015613
International Standard Serial Number (ISSN)
- 0028-0836
Digital Object Identifier (DOI)
- 10.1038/370075a0
Language
- eng
Conference Location
- England