Frequent Imprecise Excision among Reversions of a P Element-Caused Lethal Mutation in Drosophila.

Published

Journal Article

RpII215(D) (50) (= D50) is a lethal mutation caused by the insertion of a 1.3-kb P element 5' to sequences encoding the largest (215 kilodaltons) subunit of Drosophila RNA polymerase II. In dysgenic males D50 reverted to nonlethality at frequencies ranging from 2.6 to 6.5%. These reversions resulted from loss of P element sequences. Genetic tests of function and restriction enzyme analysis of revertant DNAs revealed that 35% or more of the reversion events were imprecise excisions. Two meiotic mutations that perturb excision repair and postreplication repair (mei-9(a) and mei-41(D5), respectively) had no influence on reversion frequency but may have increased the proportion of imprecise excisions. We suggest that these excisions are by-products of, rather than intermediates in, the transposition process.

Full Text

Duke Authors

Cited Authors

  • Voelker, RA; Greenleaf, AL; Gyurkovics, H; Wisely, GB; Huang, SM; Searles, LL

Published Date

  • June 1, 1984

Published In

Volume / Issue

  • 107 / 2

Start / End Page

  • 279 - 294

PubMed ID

  • 17246216

Pubmed Central ID

  • 17246216

International Standard Serial Number (ISSN)

  • 0016-6731

Language

  • eng

Conference Location

  • United States