Liquid chromatography/tandem mass spectrometry of dolichols and polyprenols, lipid sugar carriers across evolution.


Journal Article (Review)

Across evolution, dolichols and polyprenols serve as sugar carriers in biosynthetic processes that include protein glycosylation and lipopolysaccharide biogenesis. Liquid chromatography coupled with electrospray ionization mass spectrometry offers a powerful tool for studying dolichols and polyprenols in their alcohol or glycan-modified forms in members of all three domains of life. In the following, recent examples of the how different versions of this analytical approach, namely reverse phase liquid chromatography-multiple reaction monitoring, normal phase liquid chromatography/tandem mass spectrometry and normal phase liquid chromatography-precursor ion scan detection have respectively served to address novel aspects of dolichol or polyprenol biology in Eukarya, Archaea and Bacteria.

Full Text

Duke Authors

Cited Authors

  • Guan, Z; Eichler, J

Published Date

  • November 2011

Published In

Volume / Issue

  • 1811 / 11

Start / End Page

  • 800 - 806

PubMed ID

  • 21570481

Pubmed Central ID

  • 21570481

International Standard Serial Number (ISSN)

  • 0006-3002

Digital Object Identifier (DOI)

  • 10.1016/j.bbalip.2011.04.009


  • eng

Conference Location

  • Netherlands