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A mouse macrophage lipidome.

Publication ,  Journal Article
Dennis, EA; Deems, RA; Harkewicz, R; Quehenberger, O; Brown, HA; Milne, SB; Myers, DS; Glass, CK; Hardiman, G; Reichart, D; Merrill, AH ...
Published in: J Biol Chem
December 17, 2010

We report the lipidomic response of the murine macrophage RAW cell line to Kdo(2)-lipid A, the active component of an inflammatory lipopolysaccharide functioning as a selective TLR4 agonist and compactin, a statin inhibitor of cholesterol biosynthesis. Analyses of lipid molecular species by dynamic quantitative mass spectrometry and concomitant transcriptomic measurements define the lipidome and demonstrate immediate responses in fatty acid metabolism represented by increases in eicosanoid synthesis and delayed responses characterized by sphingolipid and sterol biosynthesis. Lipid remodeling of glycerolipids, glycerophospholipids, and prenols also take place, indicating that activation of the innate immune system by inflammatory mediators leads to alterations in a majority of mammalian lipid categories, including unanticipated effects of a statin drug. Our studies provide a systems-level view of lipid metabolism and reveal significant connections between lipid and cell signaling and biochemical pathways that contribute to innate immune responses and to pharmacological perturbations.

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Published In

J Biol Chem

DOI

EISSN

1083-351X

Publication Date

December 17, 2010

Volume

285

Issue

51

Start / End Page

39976 / 39985

Location

United States

Related Subject Headings

  • Toll-Like Receptor 4
  • Mice
  • Macrophages
  • Lipopolysaccharides
  • Lipid Metabolism
  • Inflammation Mediators
  • Immunity, Innate
  • Cell Line
  • Biochemistry & Molecular Biology
  • Animals
 

Citation

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Dennis, E. A., Deems, R. A., Harkewicz, R., Quehenberger, O., Brown, H. A., Milne, S. B., … Subramaniam, S. (2010). A mouse macrophage lipidome. J Biol Chem, 285(51), 39976–39985. https://doi.org/10.1074/jbc.M110.182915
Dennis, Edward A., Raymond A. Deems, Richard Harkewicz, Oswald Quehenberger, H Alex Brown, Stephen B. Milne, David S. Myers, et al. “A mouse macrophage lipidome.J Biol Chem 285, no. 51 (December 17, 2010): 39976–85. https://doi.org/10.1074/jbc.M110.182915.
Dennis EA, Deems RA, Harkewicz R, Quehenberger O, Brown HA, Milne SB, et al. A mouse macrophage lipidome. J Biol Chem. 2010 Dec 17;285(51):39976–85.
Dennis, Edward A., et al. “A mouse macrophage lipidome.J Biol Chem, vol. 285, no. 51, Dec. 2010, pp. 39976–85. Pubmed, doi:10.1074/jbc.M110.182915.
Dennis EA, Deems RA, Harkewicz R, Quehenberger O, Brown HA, Milne SB, Myers DS, Glass CK, Hardiman G, Reichart D, Merrill AH, Sullards MC, Wang E, Murphy RC, Raetz CRH, Garrett TA, Guan Z, Ryan AC, Russell DW, McDonald JG, Thompson BM, Shaw WA, Sud M, Zhao Y, Gupta S, Maurya MR, Fahy E, Subramaniam S. A mouse macrophage lipidome. J Biol Chem. 2010 Dec 17;285(51):39976–39985.

Published In

J Biol Chem

DOI

EISSN

1083-351X

Publication Date

December 17, 2010

Volume

285

Issue

51

Start / End Page

39976 / 39985

Location

United States

Related Subject Headings

  • Toll-Like Receptor 4
  • Mice
  • Macrophages
  • Lipopolysaccharides
  • Lipid Metabolism
  • Inflammation Mediators
  • Immunity, Innate
  • Cell Line
  • Biochemistry & Molecular Biology
  • Animals