Identification and quantification of dolichol and dolichoic acid in neuromelanin from substantia nigra of the human brain.

Published

Journal Article

Neuromelanin (NM) isolated from the substantia nigra of the human brain is found to contain a series of dolichoic acids (dol-CA) containing 14-20 isoprene units. This is the first observation of dol-CA in a natural system. Using internally spiked nor-dolichol and nor-dolichoic acid standards, the concentrations of dolichol (dol) and dol-CA present in NM were determined. Remarkably, dol was only four times as abundant as dol-CA in NM. The distribution of dol-CA chains lengths in NM also differed from that of dol, suggesting that the enzyme(s) responsible for the conversion of dol to dol-CA prefer a dolichol substrate containing 19 isoprene units.

Full Text

Duke Authors

Cited Authors

  • Ward, WC; Guan, Z; Zucca, FA; Fariello, RG; Kordestani, R; Zecca, L; Raetz, CRH; Simon, JD

Published Date

  • July 2007

Published In

Volume / Issue

  • 48 / 7

Start / End Page

  • 1457 - 1462

PubMed ID

  • 17446624

Pubmed Central ID

  • 17446624

International Standard Serial Number (ISSN)

  • 0022-2275

Digital Object Identifier (DOI)

  • 10.1194/jlr.C700008-JLR200

Language

  • eng

Conference Location

  • United States