Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli.

Published

Journal Article

Undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc (lipid II) is extracted from Escherichia coli cells by utilizing its unusual pH-dependent solubility property in a Bligh-Dyer system, and identified by electrospray ionization mass spectrometry in conjunction with a novel 15N mass shift analysis. The described approach will facilitate the structural characterization of lipid II variants from diverse bacteria, including antibiotic-resistant mutants, as well as the numerous minor uncharacterized lipids present in all biological systems.

Full Text

Duke Authors

Cited Authors

  • Guan, Z; Breazeale, SD; Raetz, CRH

Published Date

  • October 2005

Published In

Volume / Issue

  • 345 / 2

Start / End Page

  • 336 - 339

PubMed ID

  • 16118008

Pubmed Central ID

  • 16118008

Electronic International Standard Serial Number (EISSN)

  • 1096-0309

International Standard Serial Number (ISSN)

  • 0003-2697

Digital Object Identifier (DOI)

  • 10.1016/j.ab.2005.07.002

Language

  • eng