Electron capture dissociation mass spectrometry in characterization of post-translational modifications.

Published

Journal Article (Review)

Electron capture dissociation (ECD) represents a significant advance in tandem mass spectrometry for the identification and characterization of post-translational modifications (PTMs) of polypeptides. In comparison with the conventional fragmentation techniques, such as collisionally induced dissociation and infrared multi-photon dissociation, ECD provides more extensive sequence fragments, while allowing the labile modifications to remain intact during backbone fragmentation. This unique attribute offers ECD as an attractive alternative for detection and localization of PTMs. The success and rapid adoption of ECD recently led to the culmination of The 1st International Uppsala Symposium on Electron Capture Dissociation of Biomolecules and Related Phenomena (October 19-22, 2003, Stockholm, Sweden). Herein, we present a general overview of the ECD technique as well as selected applications in characterization of post-translationally modified polypeptides.

Full Text

Duke Authors

Cited Authors

  • Bakhtiar, R; Guan, Z

Published Date

  • August 2005

Published In

Volume / Issue

  • 334 / 1

Start / End Page

  • 1 - 8

PubMed ID

  • 15950932

Pubmed Central ID

  • 15950932

Electronic International Standard Serial Number (EISSN)

  • 1090-2104

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/j.bbrc.2005.05.138

Language

  • eng