Three-dimensional culture systems to induce chondrogenesis of adipose-derived stem cells.


Journal Article

Stem cells can easily be harvested from adipose tissue in large numbers for use in tissue-engineering approaches for cartilage repair or regeneration. In this chapter, we describe in vitro tissue-engineering models that we have used in our laboratory for the chondrogenic induction of adipose-derived stem cells (ASC). In addition to the proper growth factor environment, chondrogenesis requires cells to be maintained in a rounded morphology in three-dimensional (3D) culture, and thus properties of the biomaterial scaffold also play a critical role in ASC differentiation. Histologic and immunohistologic methods for assessing chondrogenesis are also presented. In general, 10-12 weeks are required to assess ASC chondrogenesis in these model systems.

Full Text

Cited Authors

  • Estes, BT; Guilak, F

Published Date

  • January 2011

Published In

Volume / Issue

  • 702 /

Start / End Page

  • 201 - 217

PubMed ID

  • 21082404

Pubmed Central ID

  • 21082404

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1007/978-1-61737-960-4_15


  • eng