Engineered cartilage using primary chondrocytes cultured in a porous cartilage-derived matrix.

Published

Journal Article

To investigate the cell growth, matrix accumulation and mechanical properties of neocartilage formed by human or porcine articular chondrocytes on a porous, porcine cartilage-derived matrix (CDM) for use in cartilage tissue engineering.We examined the physical properties, cell infiltration and matrix accumulation in different formulations of CDM and selected a CDM made of homogenized cartilage slurry as an appropriate scaffold for long-term culture of human and porcine articular chondrocytes.The CDM scaffold supported growth and proliferation of both human and porcine chondrocytes. Histology and immunohistochemistry showed abundant cartilage-specific macromolecule deposition at day 28. Human chondrocytes migrated throughout the CDM, showing a relatively homogeneous distribution of new tissue accumulation, whereas porcine chondrocytes tended to form a proteoglycan-rich layer primarily on the surfaces of the scaffold. Human chondrocyte-seeded scaffolds had a significantly lower aggregate modulus and hydraulic permeability at day 28.These data show that a scaffold derived from native porcine articular cartilage can support neocartilage formation in the absence of exogenous growth factors. The overall characteristics and properties of the constructs depend on factors such as the concentration of CDM used, the porosity of the scaffold, and the species of chondrocytes.

Full Text

Cited Authors

  • Cheng, N-C; Estes, BT; Young, T-H; Guilak, F

Published Date

  • January 2011

Published In

Volume / Issue

  • 6 / 1

Start / End Page

  • 81 - 93

PubMed ID

  • 21175289

Pubmed Central ID

  • 21175289

Electronic International Standard Serial Number (EISSN)

  • 1746-076X

International Standard Serial Number (ISSN)

  • 1746-0751

Digital Object Identifier (DOI)

  • 10.2217/rme.10.87

Language

  • eng