Multifunctional hybrid three-dimensionally woven scaffolds for cartilage tissue engineering.
Published
Journal Article
The successful replacement of large-scale cartilage defects or osteoarthritic lesions using tissue-engineering approaches will likely require composite biomaterial scaffolds that have biomimetic mechanical properties and can provide cell-instructive cues to control the growth and differentiation of embedded stem or progenitor cells. This study describes a novel method of constructing multifunctional scaffolds for cartilage tissue engineering that can provide both mechanical support and biological stimulation to seeded progenitor cells. 3-D woven PCL scaffolds were infiltrated with a slurry of homogenized CDM of porcine origin, seeded with human ASCs, and cultured for up to 42 d under standard growth conditions. These constructs were compared to scaffolds derived solely from CDM as well as 3-D woven PCL fabric without CDM. While all scaffolds promoted a chondrogenic phenotype of the ASCs, CDM scaffolds showed low compressive and shear moduli and contracted significantly during culture. Fiber-reinforced CDM scaffolds and 3-D woven PCL scaffolds maintained their mechanical properties throughout the culture period, while supporting the accumulation of a cartilaginous extracellular matrix. These findings show that fiber-reinforced hybrid scaffolds can be produced with biomimetic mechanical properties as well as the ability to promote ASC differentiation and chondrogenesis in vitro.
Full Text
Cited Authors
- Moutos, FT; Estes, BT; Guilak, F
Published Date
- November 2010
Published In
Volume / Issue
- 10 / 11
Start / End Page
- 1355 - 1364
PubMed ID
- 20857388
Pubmed Central ID
- 20857388
Electronic International Standard Serial Number (EISSN)
- 1616-5195
International Standard Serial Number (ISSN)
- 1616-5187
Digital Object Identifier (DOI)
- 10.1002/mabi.201000124
Language
- eng