Multifunctional hybrid three-dimensionally woven scaffolds for cartilage tissue engineering.

Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't

The successful replacement of large-scale cartilage defects or osteoarthritic lesions using tissue-engineering approaches will likely require composite biomaterial scaffolds that have biomimetic mechanical properties and can provide cell-instructive cues to control the growth and differentiation of embedded stem or progenitor cells. This study describes a novel method of constructing multifunctional scaffolds for cartilage tissue engineering that can provide both mechanical support and biological stimulation to seeded progenitor cells. 3-D woven PCL scaffolds were infiltrated with a slurry of homogenized CDM of porcine origin, seeded with human ASCs, and cultured for up to 42 d under standard growth conditions. These constructs were compared to scaffolds derived solely from CDM as well as 3-D woven PCL fabric without CDM. While all scaffolds promoted a chondrogenic phenotype of the ASCs, CDM scaffolds showed low compressive and shear moduli and contracted significantly during culture. Fiber-reinforced CDM scaffolds and 3-D woven PCL scaffolds maintained their mechanical properties throughout the culture period, while supporting the accumulation of a cartilaginous extracellular matrix. These findings show that fiber-reinforced hybrid scaffolds can be produced with biomimetic mechanical properties as well as the ability to promote ASC differentiation and chondrogenesis in vitro.

Full Text

Duke Authors

Cited Authors

  • Moutos, FT; Estes, BT; Guilak, F

Published Date

  • November 10, 2010

Published In

Volume / Issue

  • 10 / 11

Start / End Page

  • 1355 - 1364

PubMed ID

  • 20857388

Electronic International Standard Serial Number (EISSN)

  • 1616-5195

Digital Object Identifier (DOI)

  • 10.1002/mabi.201000124

Language

  • eng

Citation Source

  • PubMed