Ligament-derived matrix stimulates a ligamentous phenotype in human adipose-derived stem cells.

Journal Article (Journal Article)

Human adipose stem cells (hASCs) can differentiate into a variety of phenotypes. Native extracellular matrix (e.g., demineralized bone matrix or small intestinal submucosa) can influence the growth and differentiation of stem cells. The hypothesis of this study was that a novel ligament-derived matrix (LDM) would enhance expression of a ligamentous phenotype in hASCs compared to collagen gel alone. LDM prepared using phosphate-buffered saline or 0.1% peracetic acid was mixed with collagen gel (COL) and was evaluated for its ability to induce proliferation, differentiation, and extracellular matrix synthesis in hASCs over 28 days in culture at different seeding densities (0, 0.25 x 10(6), 1 x 10(6), or 2 x 10(6) hASC/mL). Biochemical and gene expression data were analyzed using analysis of variance. Fisher's least significant difference test was used to determine differences between treatments following analysis of variance. hASCs in either LDM or COL demonstrated changes in gene expression consistent with ligament development. hASCs cultured with LDM demonstrated more dsDNA content, sulfated-glycosaminoglycan accumulation, and type I and III collagen synthesis, and released more sulfated-glycosaminoglycan and collagen into the medium compared to hASCs in COL (p

Full Text

Duke Authors

Cited Authors

  • Little, D; Guilak, F; Ruch, DS

Published Date

  • July 2010

Published In

Volume / Issue

  • 16 / 7

Start / End Page

  • 2307 - 2319

PubMed ID

  • 20406104

Pubmed Central ID

  • PMC2947935

Electronic International Standard Serial Number (EISSN)

  • 1937-335X

Digital Object Identifier (DOI)

  • 10.1089/ten.tea.2009.0720


  • eng

Conference Location

  • United States