[Murine Doube Minute 2 oncogene inhibited apoptosis induced by retroviral-mediated wild-type p53].
OBJECTIVE: To investigate the interaction between Murine Doube Minute 2 (MDM2) oncogene and p53 gene in the evolution of apoptosis in pancreatic carcinoma. METHODS: A recombinant retroviral vector expressing wild-type p53 was constructed and packaged by packaging cell line PA317 cells using calcium phosphate coprecipitation method. The viral supernatant of the packaged vector was used to transfect the pancreatic carcinoma cell line(PC-2), a transformant cell line PC-2/swtp53 was then established. A recombinant vector pCMV-MDM2 was transduced into PC-2/swtp53 cells by lipofectin-mediated method, a double transfected cell line PC-2/swtp53/pCMV-MDM2 was formed. RESULTS: By means of flow cytometry, in situ TdT analysis and DNA agarose gel electrophoresis, an increase of apoptosis was demonstrated in PC-2/swtp53 cell line (> 12%), whereas apoptosis was decreased in PC-2/swtp53/pCMV-MDM2 cell line (3.2%). CONCLUSIONS: MDM2 oncogene can inhibit apoptosis induced by wild-type p53 gene in pancreatic carcinoma.
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