[The effect of adenovirus-mediated wild-type p53 expression on the growth of human pancreatic carcinoma cell line cells].
(English Abstract;Journal Article)
OBJECTIVE: To evaluate the effect of the wild-type p53(wtp53) expression on the growth of pancreatic carcinoma cell line. METHODS: A recombinant adenovirus vector (E1 minus)-Ad5CMVwtp53 expressing human wild-type p53 was constructed, the vector contained a human CMV promoter, a human wild-type p53 cDNA and SV40 polyadenylation signal. The Ad5CMVwtp53 and control adenoviral vector Ad5pXJ were amplified in 293 packaging cells and were used to transfect human pancreatic carcinoma cell line (PC-2). The presence and expression of wtp53 in the cells transfected with Ad5CMVwtp53 were confirmed by PCR and Northern blot. RESULTS: The transformant cells exhibited growth retardation and lost the ability to form colony in soft agar. CONCLUSIONS: The restoration of wtp53 expression in human pancreatic carcinoma cells which express endogenous mutant p53, can suppress the growth and partly reverse the malignant phenotype of pancreatic carcinoma cells.
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