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Cell cycle analysis of budding yeast using SYTOX Green.

Publication ,  Journal Article
Haase, SB
Published in: Current protocols in cytometry
November 2004

Flow cytometric determination of DNA content is a standard tool for cell cycle analysis in the budding yeast, Saccharomyces cerevisiae. Using SYTOX Green instead of propidium iodide (PI) in a standard ethanol fixation protocol increases the accuracy and reproducibility of cell cycle analyses. SYTOX-stained cells exhibit improved coefficients of variation (CVs), a better correlation between DNA content and fluorescence, and decreased sensitivity to variances in experimental conditions when compared with PI-stained cells. The SYTOX Green protocol provides researchers with a more accurate and reliable alternative to standard PI-based protocols for the analysis of the budding yeast cell cycle.

Duke Scholars

Published In

Current protocols in cytometry

DOI

EISSN

1934-9300

ISSN

1934-9297

Publication Date

November 2004

Volume

Chapter 7

Start / End Page

Unit / 7.23

Related Subject Headings

  • Saccharomycetales
  • Organic Chemicals
  • Methods
  • Indicators and Reagents
  • Humans
  • Flow Cytometry
  • DNA
  • Cell Cycle
 

Citation

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Chicago
ICMJE
MLA
NLM
Haase, S. B. (2004). Cell cycle analysis of budding yeast using SYTOX Green. Current Protocols in Cytometry, Chapter 7, Unit-7.23. https://doi.org/10.1002/0471142956.cy0723s26
Haase, Steven B. “Cell cycle analysis of budding yeast using SYTOX Green.Current Protocols in Cytometry Chapter 7 (November 2004): Unit-7.23. https://doi.org/10.1002/0471142956.cy0723s26.
Haase SB. Cell cycle analysis of budding yeast using SYTOX Green. Current protocols in cytometry. 2004 Nov;Chapter 7:Unit-7.23.
Haase, Steven B. “Cell cycle analysis of budding yeast using SYTOX Green.Current Protocols in Cytometry, vol. Chapter 7, Nov. 2004, p. Unit-7.23. Epmc, doi:10.1002/0471142956.cy0723s26.
Haase SB. Cell cycle analysis of budding yeast using SYTOX Green. Current protocols in cytometry. 2004 Nov;Chapter 7:Unit-7.23.

Published In

Current protocols in cytometry

DOI

EISSN

1934-9300

ISSN

1934-9297

Publication Date

November 2004

Volume

Chapter 7

Start / End Page

Unit / 7.23

Related Subject Headings

  • Saccharomycetales
  • Organic Chemicals
  • Methods
  • Indicators and Reagents
  • Humans
  • Flow Cytometry
  • DNA
  • Cell Cycle