Cell cycle analysis of budding yeast using SYTOX Green.

Published

Journal Article

Flow cytometric determination of DNA content is a standard tool for cell cycle analysis in the budding yeast, Saccharomyces cerevisiae. Using SYTOX Green instead of propidium iodide (PI) in a standard ethanol fixation protocol increases the accuracy and reproducibility of cell cycle analyses. SYTOX-stained cells exhibit improved coefficients of variation (CVs), a better correlation between DNA content and fluorescence, and decreased sensitivity to variances in experimental conditions when compared with PI-stained cells. The SYTOX Green protocol provides researchers with a more accurate and reliable alternative to standard PI-based protocols for the analysis of the budding yeast cell cycle.

Full Text

Duke Authors

Cited Authors

  • Haase, SB

Published Date

  • November 2004

Published In

Volume / Issue

  • Chapter 7 /

Start / End Page

  • Unit - 7.23

PubMed ID

  • 18770800

Pubmed Central ID

  • 18770800

Electronic International Standard Serial Number (EISSN)

  • 1934-9300

International Standard Serial Number (ISSN)

  • 1934-9297

Digital Object Identifier (DOI)

  • 10.1002/0471142956.cy0723s26

Language

  • eng