High-speed mapping of synaptic connectivity using photostimulation in Channelrhodopsin-2 transgenic mice.


Journal Article

To permit rapid optical control of brain activity, we have engineered multiple lines of transgenic mice that express the light-activated cation channel Channelrhodopsin-2 (ChR2) in subsets of neurons. Illumination of ChR2-positive neurons in brain slices produced photocurrents that generated action potentials within milliseconds and with precisely timed latencies. The number of light-evoked action potentials could be controlled by varying either the amplitude or duration of illumination. Furthermore, the frequency of light-evoked action potentials could be precisely controlled up to 30 Hz. Photostimulation also could evoke synaptic transmission between neurons, and, by scanning with a small laser light spot, we were able to map the spatial distribution of synaptic circuits connecting neurons within living cerebral cortex. We conclude that ChR2 is a genetically based photostimulation technology that permits analysis of neural circuits with high spatial and temporal resolution in transgenic mammals.

Full Text

Duke Authors

Cited Authors

  • Wang, H; Peca, J; Matsuzaki, M; Matsuzaki, K; Noguchi, J; Qiu, L; Wang, D; Zhang, F; Boyden, E; Deisseroth, K; Kasai, H; Hall, WC; Feng, G; Augustine, GJ

Published Date

  • May 8, 2007

Published In

Volume / Issue

  • 104 / 19

Start / End Page

  • 8143 - 8148

PubMed ID

  • 17483470

Pubmed Central ID

  • 17483470

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0700384104


  • eng

Conference Location

  • United States