High-speed mapping of synaptic connectivity using photostimulation in Channelrhodopsin-2 transgenic mice.
To permit rapid optical control of brain activity, we have engineered multiple lines of transgenic mice that express the light-activated cation channel Channelrhodopsin-2 (ChR2) in subsets of neurons. Illumination of ChR2-positive neurons in brain slices produced photocurrents that generated action potentials within milliseconds and with precisely timed latencies. The number of light-evoked action potentials could be controlled by varying either the amplitude or duration of illumination. Furthermore, the frequency of light-evoked action potentials could be precisely controlled up to 30 Hz. Photostimulation also could evoke synaptic transmission between neurons, and, by scanning with a small laser light spot, we were able to map the spatial distribution of synaptic circuits connecting neurons within living cerebral cortex. We conclude that ChR2 is a genetically based photostimulation technology that permits analysis of neural circuits with high spatial and temporal resolution in transgenic mammals.
Wang, H; Peca, J; Matsuzaki, M; Matsuzaki, K; Noguchi, J; Qiu, L; Wang, D; Zhang, F; Boyden, E; Deisseroth, K; Kasai, H; Hall, WC; Feng, G; Augustine, GJ
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