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Excitatory and inhibitory circuitry in the superficial gray layer of the superior colliculus.

Publication ,  Journal Article
Lee, PH; Schmidt, M; Hall, WC
Published in: J Neurosci
October 15, 2001

Stratum griseum superficiale (SGS) of the superior colliculus receives a dense cholinergic input from the parabigeminal nucleus. In this study, we examined in vitro the modulatory influence of acetylcholine (ACh) on the responses of SGS neurons that project to the visual thalamus in the rat. We used whole-cell patch-clamp recording to measure the responses of these projection neurons to electrical stimulation of their afferents in the stratum opticum (SO) before and during local pressure injections of ACh. These colliculothalamic projection neurons (CTNs) were identified during the in vitro experiments by prelabeling them from the thalamus with the retrograde axonal tracer wheat germ agglutinin-apo-HRP-gold. In a group of cells that included the prelabeled neurons, EPSCs evoked by SO stimulation were significantly reduced by the application of ACh, whereas IPSC amplitudes were significantly enhanced. Similar effects were observed when the nicotinic ACh receptor agonist lobeline was used. Application of the selective GABA(B) receptor antagonist 3-[[(3,4-dichlorophenyl)-methyl]amino]propyl](diethoxymethyl)phosphinic acid blocked ACh-induced reduction in the evoked response. In contrast, the ACh-induced reduction was insensitive to application of the GABA(A) receptor antagonist bicuculline. The ACh-induced reduction was also diminished by bath application of muscimol at the low concentrations that selectively activate GABA(C) receptors. Because GABA(C) receptors may be specifically expressed by GABAergic SGS interneurons (Schmidt et al., 2001), our results support the hypothesis that ACh reduces CTN activity by nicotinic receptor-mediated excitation of local GABAergic interneurons. These interneurons in turn use GABA(B) receptors to inhibit the CTNs.

Duke Scholars

Published In

J Neurosci

DOI

EISSN

1529-2401

Publication Date

October 15, 2001

Volume

21

Issue

20

Start / End Page

8145 / 8153

Location

United States

Related Subject Headings

  • Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
  • Visual Pathways
  • Thalamus
  • Superior Colliculi
  • Rats, Wistar
  • Rats
  • Patch-Clamp Techniques
  • Nicotinic Agonists
  • Neurology & Neurosurgery
  • Neural Inhibition
 

Citation

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MLA
NLM
Lee, P. H., Schmidt, M., & Hall, W. C. (2001). Excitatory and inhibitory circuitry in the superficial gray layer of the superior colliculus. J Neurosci, 21(20), 8145–8153. https://doi.org/10.1523/JNEUROSCI.21-20-08145.2001
Lee, P. H., M. Schmidt, and W. C. Hall. “Excitatory and inhibitory circuitry in the superficial gray layer of the superior colliculus.J Neurosci 21, no. 20 (October 15, 2001): 8145–53. https://doi.org/10.1523/JNEUROSCI.21-20-08145.2001.
Lee PH, Schmidt M, Hall WC. Excitatory and inhibitory circuitry in the superficial gray layer of the superior colliculus. J Neurosci. 2001 Oct 15;21(20):8145–53.
Lee, P. H., et al. “Excitatory and inhibitory circuitry in the superficial gray layer of the superior colliculus.J Neurosci, vol. 21, no. 20, Oct. 2001, pp. 8145–53. Pubmed, doi:10.1523/JNEUROSCI.21-20-08145.2001.
Lee PH, Schmidt M, Hall WC. Excitatory and inhibitory circuitry in the superficial gray layer of the superior colliculus. J Neurosci. 2001 Oct 15;21(20):8145–8153.

Published In

J Neurosci

DOI

EISSN

1529-2401

Publication Date

October 15, 2001

Volume

21

Issue

20

Start / End Page

8145 / 8153

Location

United States

Related Subject Headings

  • Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
  • Visual Pathways
  • Thalamus
  • Superior Colliculi
  • Rats, Wistar
  • Rats
  • Patch-Clamp Techniques
  • Nicotinic Agonists
  • Neurology & Neurosurgery
  • Neural Inhibition