Isolation of renal brush borders.

Published

Journal Article

Methods are described to isolate intact brush borders and brush border membranes from renal cell homogenates. A rapid method yields sealed vesicles that reconstitute renal brush border transport. In one variation of this protocol, 10 to 20 mM CaCl2 or MgCl2 is added to aggregate non-brush border structures for subsequent removal by centrifugation. For analytical studies, guidance is provided for subsequent purification steps including preparative free-flow and aqueous two-phase partition. Marker enzymes and morphological parameters are included for assessment of yield and fraction purity.

Full Text

Duke Authors

Cited Authors

  • Morré, DJ; Hammond, T

Published Date

  • March 2007

Published In

Volume / Issue

  • Chapter 3 /

Start / End Page

  • Unit - 3.26

PubMed ID

  • 18228514

Pubmed Central ID

  • 18228514

Electronic International Standard Serial Number (EISSN)

  • 1934-2616

Digital Object Identifier (DOI)

  • 10.1002/0471143030.cb0326s34

Language

  • eng

Conference Location

  • United States