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Saccharomyces cerevisiae gene expression changes during rotating wall vessel suspension culture.

Publication ,  Journal Article
Johanson, K; Allen, PL; Lewis, F; Cubano, LA; Hyman, LE; Hammond, TG
Published in: J Appl Physiol (1985)
December 2002

This study utilizes Saccharomyces cerevisiae to study genetic responses to suspension culture. The suspension culture system used in this study is the high-aspect-ratio vessel, one type of the rotating wall vessel, that provides a high rate of gas exchange necessary for rapidly dividing cells. Cells were grown in the high-aspect-ratio vessel, and DNA microarray and metabolic analyses were used to determine the resulting changes in yeast gene expression. A significant number of genes were found to be up- or downregulated by at least twofold as a result of rotational growth. By using Gibbs promoter alignment, clusters of genes were examined for promoter elements mediating these genetic changes. Candidate binding motifs similar to the Rap1p binding site and the stress-responsive element were identified in the promoter regions of differentially regulated genes. This study shows that, as in higher order organisms, S. cerevisiae changes gene expression in response to rotational culture and also provides clues for investigations into the signaling pathways involved in gravitational response.

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Published In

J Appl Physiol (1985)

DOI

ISSN

8750-7587

Publication Date

December 2002

Volume

93

Issue

6

Start / End Page

2171 / 2180

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Rotation
  • Promoter Regions, Genetic
  • Physiology
  • Oligonucleotide Array Sequence Analysis
  • Gravitation
  • Gene Expression Regulation, Fungal
  • Cluster Analysis
  • Blotting, Northern
  • 42 Health sciences
 

Citation

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Johanson, K., Allen, P. L., Lewis, F., Cubano, L. A., Hyman, L. E., & Hammond, T. G. (2002). Saccharomyces cerevisiae gene expression changes during rotating wall vessel suspension culture. J Appl Physiol (1985), 93(6), 2171–2180. https://doi.org/10.1152/japplphysiol.01087.2001
Johanson, Kelly, Patricia L. Allen, Fawn Lewis, Luis A. Cubano, Linda E. Hyman, and Timothy G. Hammond. “Saccharomyces cerevisiae gene expression changes during rotating wall vessel suspension culture.J Appl Physiol (1985) 93, no. 6 (December 2002): 2171–80. https://doi.org/10.1152/japplphysiol.01087.2001.
Johanson K, Allen PL, Lewis F, Cubano LA, Hyman LE, Hammond TG. Saccharomyces cerevisiae gene expression changes during rotating wall vessel suspension culture. J Appl Physiol (1985). 2002 Dec;93(6):2171–80.
Johanson, Kelly, et al. “Saccharomyces cerevisiae gene expression changes during rotating wall vessel suspension culture.J Appl Physiol (1985), vol. 93, no. 6, Dec. 2002, pp. 2171–80. Pubmed, doi:10.1152/japplphysiol.01087.2001.
Johanson K, Allen PL, Lewis F, Cubano LA, Hyman LE, Hammond TG. Saccharomyces cerevisiae gene expression changes during rotating wall vessel suspension culture. J Appl Physiol (1985). 2002 Dec;93(6):2171–2180.

Published In

J Appl Physiol (1985)

DOI

ISSN

8750-7587

Publication Date

December 2002

Volume

93

Issue

6

Start / End Page

2171 / 2180

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Rotation
  • Promoter Regions, Genetic
  • Physiology
  • Oligonucleotide Array Sequence Analysis
  • Gravitation
  • Gene Expression Regulation, Fungal
  • Cluster Analysis
  • Blotting, Northern
  • 42 Health sciences