Mast cell regulation of inflammation and gene expression during antigen-induced bladder inflammation in mice.

Journal Article (Journal Article)

Mast cell numbers are significantly increased in bladder disorders including malignancy and interstitial cystitis, but their precise role has been difficult to determine. We characterized the role of mast cells on gene regulation associated with antigen-induced bladder inflammation in mice. For this purpose, we examined the responses in mast cell-deficient (Kit(W)/Kit(W-v)), congenic normal (+/+), and Kit(W)/Kit(W-v) mice that were reconstituted with bone marrow stem cells (BMR) to restore mast cells. All mice were actively sensitized and challenged intravesically with either saline or specific antigen. Bladder inflammation occurred in +/+ and BMR but not the Kit(W)/Kit(W-v) mice. Gene expression was determined using mouse cDNA expression arrays. Self-organizing maps, performed without preconditions, indicated gene expression changes dependent on the presence of mast cells. These genes were upregulated in bladders isolated from antigen challenge of +/+, not altered in Kit(W)/Kit(W-v), and were upregulated in BMR mice. Taken together these results demonstrate an important role for mast cells in allergic cystitis and indicate that mast cells can alter their environment by regulating tissue gene expression.

Full Text

Duke Authors

Cited Authors

  • Saban, R; Saban, MR; Nguyen, NB; Hammond, TG; Wershil, BK

Published Date

  • October 10, 2001

Published In

Volume / Issue

  • 7 / 1

Start / End Page

  • 35 - 43

PubMed ID

  • 11595790

Electronic International Standard Serial Number (EISSN)

  • 1531-2267

Digital Object Identifier (DOI)

  • 10.1152/physiolgenomics.00044.2001


  • eng

Conference Location

  • United States