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LPS-sensory peptide communication in experimental cystitis.

Publication ,  Journal Article
Saban, MR; Saban, R; Hammond, TG; Haak-Frendscho, M; Steinberg, H; Tengowski, MW; Bjorling, DE
Published in: Am J Physiol Renal Physiol
February 2002

Stimulation of sensory nerves can lead to release of peptides such as substance P (SP) and consequently to neurogenic inflammation. We studied the role of bacterial lipopolysaccharide (LPS) in regulating SP-induced inflammation. Experimental cystitis was induced in female mice by intravesical instillation of SP, LPS, or fluorescein-labeled LPS. Uptake of fluorescein-labeled LPS was determined by confocal analysis, and bladder inflammation was determined by morphological analysis. SP was infused into the bladders of some mice 24 h after exposure to LPS. In vitro studies determined the capacity of LPS and SP to induce histamine and cytokine release by the bladder. LPS was taken up by urothelial cells and distributed systemically. Twenty-four hours after instillation of LPS or SP, bladder inflammation was characterized by edema and leukocytic infiltration of the bladder wall. LPS pretreatment enhanced neutrophil infiltration induced by SP, increased in vitro release of histamine, tumor necrosis factor-alpha, and interferon-gamma, and significantly reduced transforming growth factor-beta1 release. These findings suggest that LPS amplifies neurogenic inflammation, thereby playing a role in the pathogenesis of neurogenic cystitis.

Duke Scholars

Published In

Am J Physiol Renal Physiol

DOI

ISSN

1931-857X

Publication Date

February 2002

Volume

282

Issue

2

Start / End Page

F202 / F210

Location

United States

Related Subject Headings

  • Urothelium
  • Urology & Nephrology
  • Urinary Bladder
  • Tumor Necrosis Factor-alpha
  • Transforming Growth Factor beta1
  • Transforming Growth Factor beta
  • Substance P
  • Neutrophils
  • Neurons, Afferent
  • Mice, Inbred BALB C
 

Citation

APA
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ICMJE
MLA
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Saban, M. R., Saban, R., Hammond, T. G., Haak-Frendscho, M., Steinberg, H., Tengowski, M. W., & Bjorling, D. E. (2002). LPS-sensory peptide communication in experimental cystitis. Am J Physiol Renal Physiol, 282(2), F202–F210. https://doi.org/10.1152/ajprenal.0163.2001
Saban, M. R., R. Saban, T. G. Hammond, M. Haak-Frendscho, H. Steinberg, M. W. Tengowski, and D. E. Bjorling. “LPS-sensory peptide communication in experimental cystitis.Am J Physiol Renal Physiol 282, no. 2 (February 2002): F202–10. https://doi.org/10.1152/ajprenal.0163.2001.
Saban MR, Saban R, Hammond TG, Haak-Frendscho M, Steinberg H, Tengowski MW, et al. LPS-sensory peptide communication in experimental cystitis. Am J Physiol Renal Physiol. 2002 Feb;282(2):F202–10.
Saban, M. R., et al. “LPS-sensory peptide communication in experimental cystitis.Am J Physiol Renal Physiol, vol. 282, no. 2, Feb. 2002, pp. F202–10. Pubmed, doi:10.1152/ajprenal.0163.2001.
Saban MR, Saban R, Hammond TG, Haak-Frendscho M, Steinberg H, Tengowski MW, Bjorling DE. LPS-sensory peptide communication in experimental cystitis. Am J Physiol Renal Physiol. 2002 Feb;282(2):F202–F210.

Published In

Am J Physiol Renal Physiol

DOI

ISSN

1931-857X

Publication Date

February 2002

Volume

282

Issue

2

Start / End Page

F202 / F210

Location

United States

Related Subject Headings

  • Urothelium
  • Urology & Nephrology
  • Urinary Bladder
  • Tumor Necrosis Factor-alpha
  • Transforming Growth Factor beta1
  • Transforming Growth Factor beta
  • Substance P
  • Neutrophils
  • Neurons, Afferent
  • Mice, Inbred BALB C