Mechanical culture conditions effect gene expression: gravity-induced changes on the space shuttle.

Published online

Journal Article

Three-dimensional suspension culture is a gravity-limited phenomenon. The balancing forces necessary to keep the aggregates in suspension increase directly with aggregate size. This leads to a self-propagating cycle of cell damage by balancing forces. Cell culture in microgravity avoids this trade-off. We determined which genes mediate three-dimensional culture of cell and tissue aggregates in the low-shear stress, low-turbulent environment of actual microgravity. Primary cultures of human renal cortical cells were flown on the space shuttle. Cells grown in microgravity and ground-based controls were grown for 6 days and fixed. RNA was extracted, and automated gene array analysis of the expression of 10, 000 genes was performed. A select group of genes were regulated in microgravity. These 1,632 genes were independent of known shear stress response element-dependent genes and heat shock proteins. Specific transcription factors underwent large changes in microgravity including the Wilms' tumor zinc finger protein, and the vitamin D receptor. A specific group of genes, under the control of defined transcription factors, mediate three-dimensional suspension culture under microgravity conditions.

Full Text

Duke Authors

Cited Authors

  • Hammond, TG; Benes, E; O'Reilly, KC; Wolf, DA; Linnehan, RM; Taher, A; Kaysen, JH; Allen, PL; Goodwin, TJ

Published Date

  • September 8, 2000

Published In

Volume / Issue

  • 3 / 3

Start / End Page

  • 163 - 173

PubMed ID

  • 11015612

Pubmed Central ID

  • 11015612

Electronic International Standard Serial Number (EISSN)

  • 1531-2267

Digital Object Identifier (DOI)

  • 10.1152/physiolgenomics.2000.3.3.163

Language

  • eng

Conference Location

  • United States