Skip to main content
Journal cover image

Select de novo gene and protein expression during renal epithelial cell culture in rotating wall vessels is shear stress dependent.

Publication ,  Journal Article
Kaysen, JH; Campbell, WC; Majewski, RR; Goda, FO; Navar, GL; Lewis, FC; Goodwin, TJ; Hammond, TG
Published in: J Membr Biol
March 1, 1999

The rotating wall vessel has gained popularity as a clinical cell culture tool to produce hormonal implants. It is desirable to understand the mechanisms by which the rotating wall vessel induces genetic changes, if we are to prolong the useful life of implants. During rotating wall vessel culture gravity is balanced by equal and opposite hydrodynamic forces including shear stress. The current study provides the first evidence that shear stress response elements, which modulate gene expression in endothelial cells, are also active in epithelial cells. Rotating wall culture of renal cells changes expression of select gene products including the giant glycoprotein scavenger receptors cubulin and megalin, the structural microvillar protein villin, and classic shear stress response genes ICAM, VCAM and MnSOD. Using a putative endothelial cell shear stress response element binding site as a decoy, we demonstrate the role of this sequence in the regulation of selected genes in epithelial cells. However, many of the changes observed in the rotating wall vessel are independent of this response element. It remains to define other genetic response elements modulated during rotating wall vessel culture, including the role of hemodynamics characterized by 3-dimensionality, low shear and turbulence, and cospatial relation of dissimilar cell types.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Membr Biol

DOI

ISSN

0022-2631

Publication Date

March 1, 1999

Volume

168

Issue

1

Start / End Page

77 / 89

Location

United States

Related Subject Headings

  • Superoxide Dismutase
  • Stress, Mechanical
  • Rotation
  • Reverse Transcriptase Polymerase Chain Reaction
  • Receptors, Cell Surface
  • Rats, Sprague-Dawley
  • Rats
  • Prostheses and Implants
  • Physiology
  • Oligonucleotides, Antisense
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kaysen, J. H., Campbell, W. C., Majewski, R. R., Goda, F. O., Navar, G. L., Lewis, F. C., … Hammond, T. G. (1999). Select de novo gene and protein expression during renal epithelial cell culture in rotating wall vessels is shear stress dependent. J Membr Biol, 168(1), 77–89. https://doi.org/10.1007/s002329900499
Kaysen, J. H., W. C. Campbell, R. R. Majewski, F. O. Goda, G. L. Navar, F. C. Lewis, T. J. Goodwin, and T. G. Hammond. “Select de novo gene and protein expression during renal epithelial cell culture in rotating wall vessels is shear stress dependent.J Membr Biol 168, no. 1 (March 1, 1999): 77–89. https://doi.org/10.1007/s002329900499.
Kaysen JH, Campbell WC, Majewski RR, Goda FO, Navar GL, Lewis FC, et al. Select de novo gene and protein expression during renal epithelial cell culture in rotating wall vessels is shear stress dependent. J Membr Biol. 1999 Mar 1;168(1):77–89.
Kaysen, J. H., et al. “Select de novo gene and protein expression during renal epithelial cell culture in rotating wall vessels is shear stress dependent.J Membr Biol, vol. 168, no. 1, Mar. 1999, pp. 77–89. Pubmed, doi:10.1007/s002329900499.
Kaysen JH, Campbell WC, Majewski RR, Goda FO, Navar GL, Lewis FC, Goodwin TJ, Hammond TG. Select de novo gene and protein expression during renal epithelial cell culture in rotating wall vessels is shear stress dependent. J Membr Biol. 1999 Mar 1;168(1):77–89.
Journal cover image

Published In

J Membr Biol

DOI

ISSN

0022-2631

Publication Date

March 1, 1999

Volume

168

Issue

1

Start / End Page

77 / 89

Location

United States

Related Subject Headings

  • Superoxide Dismutase
  • Stress, Mechanical
  • Rotation
  • Reverse Transcriptase Polymerase Chain Reaction
  • Receptors, Cell Surface
  • Rats, Sprague-Dawley
  • Rats
  • Prostheses and Implants
  • Physiology
  • Oligonucleotides, Antisense