Skip to main content
Journal cover image

Salmonella efficiently enter and survive within cultured CD11c+ dendritic cells initiating cytokine expression.

Publication ,  Journal Article
Marriott, I; Hammond, TG; Thomas, EK; Bost, KL
Published in: Eur J Immunol
April 1999

While Salmonella infects macrophages, this cell population may not be the only one important for disseminating intracellular bacteria from mucosal sites. Dendritic cells (DC) are present in the Peyer's patches and are mobilized following stimulation. Such characteristics would seem to be ideal for the dissemination of an intracellular, mucosal pathogen. However, it has been difficult to obtain sufficient numbers of DC to assess their ability to harbor Salmonella or to monitor DC in vivo. In the present study, this problem has been addressed by expanding DC in vivo using flt3 ligand, followed by the purification of CD11c+ cells using antibody-coated magnetic beads or by fluorescence-activated cell sorting. Salmonella dublin were found to be efficiently internalized, and to survive and replicate within purified CD11c+ DC, and also in CD11c+, CD8alpha+ or CD11c+, CD11b+ DC subpopulations. The ability of Salmonella to enter DC is of similar magnitude to that reported for macrophages, suggesting that this cell population could be an important host cell for dissemination of this pathogen from mucosal sites. Furthermore, infected DC responded to Salmonella by secretion of IL-1, IL-6 and IL-12. As such, these cells may be important sources of these cytokines during the host response against Salmonella infection.

Duke Scholars

Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

April 1999

Volume

29

Issue

4

Start / End Page

1107 / 1115

Location

Germany

Related Subject Headings

  • Salmonella
  • Mice, Inbred BALB C
  • Mice
  • Integrin alphaXbeta2
  • Immunophenotyping
  • Immunology
  • Dendritic Cells
  • Cytokines
  • Cells, Cultured
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Marriott, I., Hammond, T. G., Thomas, E. K., & Bost, K. L. (1999). Salmonella efficiently enter and survive within cultured CD11c+ dendritic cells initiating cytokine expression. Eur J Immunol, 29(4), 1107–1115. https://doi.org/10.1002/(SICI)1521-4141(199904)29:04<1107::AID-IMMU1107>3.0.CO;2-0
Marriott, I., T. G. Hammond, E. K. Thomas, and K. L. Bost. “Salmonella efficiently enter and survive within cultured CD11c+ dendritic cells initiating cytokine expression.Eur J Immunol 29, no. 4 (April 1999): 1107–15. https://doi.org/10.1002/(SICI)1521-4141(199904)29:04<1107::AID-IMMU1107>3.0.CO;2-0.
Marriott I, Hammond TG, Thomas EK, Bost KL. Salmonella efficiently enter and survive within cultured CD11c+ dendritic cells initiating cytokine expression. Eur J Immunol. 1999 Apr;29(4):1107–15.
Marriott, I., et al. “Salmonella efficiently enter and survive within cultured CD11c+ dendritic cells initiating cytokine expression.Eur J Immunol, vol. 29, no. 4, Apr. 1999, pp. 1107–15. Pubmed, doi:10.1002/(SICI)1521-4141(199904)29:04<1107::AID-IMMU1107>3.0.CO;2-0.
Marriott I, Hammond TG, Thomas EK, Bost KL. Salmonella efficiently enter and survive within cultured CD11c+ dendritic cells initiating cytokine expression. Eur J Immunol. 1999 Apr;29(4):1107–1115.
Journal cover image

Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

April 1999

Volume

29

Issue

4

Start / End Page

1107 / 1115

Location

Germany

Related Subject Headings

  • Salmonella
  • Mice, Inbred BALB C
  • Mice
  • Integrin alphaXbeta2
  • Immunophenotyping
  • Immunology
  • Dendritic Cells
  • Cytokines
  • Cells, Cultured
  • Animals