Mapping of sex determination loci on the white campion (Silene latifolia) Y chromosome using amplified fragment length polymorphism.

Journal Article (Journal Article)

S. latifolia is a dioecious plant with morphologically distinct sex chromosomes. To genetically map the sex determination loci on the male-specific Y chromosome, we identified X-ray-induced sex determination mutants that had lost male traits. We used male-specific AFLP markers to characterize the extent of deletions in the Y chromosomes of the mutants. We then compared overlapping deletions to predict the order of the AFLP markers and to locate the mutated sex-determining genes. We found three regions on the Y chromosome where frequent deletions were significantly associated with loss of male traits. One was associated with hermaphroditic mutants. A second was associated with asexual mutants that lack genes needed for early stamen development and a third was associated with asexual mutants that lack genes for late stages of stamen development. Our observations confirmed a classical genetic prediction that S. latifolia has three dispersed male-determining loci on the Y chromosome, one for carpel suppression, one for early stamen development, and another for late stamen development. This AFLP map provides a framework for locating genes on the Y chromosome and for characterizing deletions on the Y chromosomes of potentially interesting mutants.

Full Text

Duke Authors

Cited Authors

  • Lebel-Hardenack, S; Hauser, E; Law, TF; Schmid, J; Grant, SR

Published Date

  • February 2002

Published In

Volume / Issue

  • 160 / 2

Start / End Page

  • 717 - 725

PubMed ID

  • 11861573

Pubmed Central ID

  • PMC1461999

International Standard Serial Number (ISSN)

  • 0016-6731

Digital Object Identifier (DOI)

  • 10.1093/genetics/160.2.717


  • eng

Conference Location

  • United States