Endothelial cell-conditioned medium downregulates smooth muscle contractile protein expression.

Published

Journal Article

Smooth muscle cells (SMC) within atherosclerotic lesions proliferate and exhibit phenotypic modulation, but the contribution of vascular endothelium to this process is poorly understood. Our aim was to examine the effects of endothelial cell-conditioned medium (ECCM) on vascular SMC growth and differentiation. Rat aortic ECCM stimulated a ninefold increase in [3H]thymidine incorporation and downregulated smooth muscle-specific myosin heavy chain and alpha-actin synthesis in rat aortic SMC. These effects were not inhibited by antibodies to platelet-derived growth factor (PDGF)-BB or PDGF-AB or with a PDGF beta-receptor subunit. Treatment with PDGF-BB (at a concentration found in ECCM), PDGF-AA, basic fibroblast growth factor, endothelin-1, or transforming growth factor-beta did not reproduce these effects. The ECCM activities were sensitive to heat and trypsinization, were >30 kDa in molecular mass, and bound weakly to heparin-Sepharose. Our data indicate that cultured endothelial cells produce a factor(s) that downregulates contractile protein expression in SMC, which may contribute to SMC dedifferentiation and proliferation.

Full Text

Duke Authors

Cited Authors

  • Vernon, SM; Campos, MJ; Haystead, T; Thompson, MM; DiCorleto, PE; Owens, GK

Published Date

  • February 1997

Published In

Volume / Issue

  • 272 / 2 Pt 1

Start / End Page

  • C582 - C591

PubMed ID

  • 9124302

Pubmed Central ID

  • 9124302

International Standard Serial Number (ISSN)

  • 0002-9513

Digital Object Identifier (DOI)

  • 10.1152/ajpcell.1997.272.2.C582

Language

  • eng

Conference Location

  • United States