Multicenter evaluation of the Staphylococcus QuickFISH method for simultaneous identification of Staphylococcus aureus and coagulase-negative staphylococci directly from blood culture bottles in less than 30 minutes.


Journal Article

A novel rapid peptide nucleic acid fluorescence in situ hybridization (FISH) method, Staphylococcus QuickFISH, for the direct detection of Staphylococcus species from positive blood culture bottles was evaluated in a multicenter clinical study. The method utilizes a microscope slide with predeposited positive- and negative-control organisms and a self-reporting 15-min hybridization step, which eliminates the need for a wash step. Five clinical laboratories tested 722 positive blood culture bottles containing gram-positive cocci in clusters. The sensitivities for detection of Staphylococcus aureus and coagulase-negative staphylococci (CoNS) were 99.5% (217/218) and 98.8% (487/493), respectively, and the combined specificity of the assay was 89.5% (17/19). The combined positive and negative predictive values of the assay were 99.7% (696/698) and 70.8% (17/24), respectively. Studies were also performed on spiked cultures to establish the specificity and performance sensitivity of the method. Staphylococcus QuickFISH has a turnaround time (TAT) of <30 min and a hands-on time (HOT) of <5 min. The ease and speed of the method have the potential to improve the accuracy of therapeutic intervention by providing S. aureus/CoNS identification simultaneously with Gram stain results.

Full Text

Cited Authors

  • Deck, MK; Anderson, ES; Buckner, RJ; Colasante, G; Coull, JM; Crystal, B; Della Latta, P; Fuchs, M; Fuller, D; Harris, W; Hazen, K; Klimas, LL; Lindao, D; Meltzer, MC; Morgan, M; Shepard, J; Stevens, S; Wu, F; Fiandaca, MJ

Published Date

  • June 2012

Published In

Volume / Issue

  • 50 / 6

Start / End Page

  • 1994 - 1998

PubMed ID

  • 22493336

Pubmed Central ID

  • 22493336

Electronic International Standard Serial Number (EISSN)

  • 1098-660X

Digital Object Identifier (DOI)

  • 10.1128/JCM.00225-12


  • eng

Conference Location

  • United States