Isolation and purification of morphogenic autoregulatory substance produced by Candida albicans.


Journal Article

The human pathogenic, dimorphic fungus Candida albicans produces in vitro a factor designated morphogenic autoregulatory substance (MARS) which suppresses yeast to mycelial transition. MARS is best produced when a high concentration of yeast cells (2 X 10(8)/ml) in a chemically defined medium is incubated at 37 degrees C for 9-15 h. MARS was isolated to apparent homogeneity by the following successive steps: charcoal absorption; pyridine elution; ether extraction; high pressure liquid chromatography, and Sephadex LH-20 chromatography. A 469-fold increase in purity was obtained by these procedures. The use of purified MARS to study C. albicans yeast to mycelial transition may lead to a better understanding of morphogeneis of this important opportunistic pathogen.

Full Text

Duke Authors

Cited Authors

  • Hazen, KC; Cutler, JE

Published Date

  • September 1, 1983

Published In

Volume / Issue

  • 94 / 3

Start / End Page

  • 777 - 783

PubMed ID

  • 6358202

Pubmed Central ID

  • 6358202

International Standard Serial Number (ISSN)

  • 0021-924X

Digital Object Identifier (DOI)

  • 10.1093/oxfordjournals.jbchem.a134419


  • eng

Conference Location

  • England