The antisense transcriptomes of human cells.


Journal Article

Transcription in mammalian cells can be assessed at a genome-wide level, but it has been difficult to reliably determine whether individual transcripts are derived from the plus or minus strands of chromosomes. This distinction can be critical for understanding the relationship between known transcripts (sense) and the complementary antisense transcripts that may regulate them. Here, we describe a technique that can be used to (i) identify the DNA strand of origin for any particular RNA transcript, and (ii) quantify the number of sense and antisense transcripts from expressed genes at a global level. We examined five different human cell types and in each case found evidence for antisense transcripts in 2900 to 6400 human genes. The distribution of antisense transcripts was distinct from that of sense transcripts, was nonrandom across the genome, and differed among cell types. Antisense transcripts thus appear to be a pervasive feature of human cells, which suggests that they are a fundamental component of gene regulation.

Full Text

Duke Authors

Cited Authors

  • He, Y; Vogelstein, B; Velculescu, VE; Papadopoulos, N; Kinzler, KW

Published Date

  • December 19, 2008

Published In

Volume / Issue

  • 322 / 5909

Start / End Page

  • 1855 - 1857

PubMed ID

  • 19056939

Pubmed Central ID

  • 19056939

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

Digital Object Identifier (DOI)

  • 10.1126/science.1163853


  • eng

Conference Location

  • United States