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The antisense transcriptomes of human cells.

Publication ,  Journal Article
He, Y; Vogelstein, B; Velculescu, VE; Papadopoulos, N; Kinzler, KW
Published in: Science
December 19, 2008

Transcription in mammalian cells can be assessed at a genome-wide level, but it has been difficult to reliably determine whether individual transcripts are derived from the plus or minus strands of chromosomes. This distinction can be critical for understanding the relationship between known transcripts (sense) and the complementary antisense transcripts that may regulate them. Here, we describe a technique that can be used to (i) identify the DNA strand of origin for any particular RNA transcript, and (ii) quantify the number of sense and antisense transcripts from expressed genes at a global level. We examined five different human cell types and in each case found evidence for antisense transcripts in 2900 to 6400 human genes. The distribution of antisense transcripts was distinct from that of sense transcripts, was nonrandom across the genome, and differed among cell types. Antisense transcripts thus appear to be a pervasive feature of human cells, which suggests that they are a fundamental component of gene regulation.

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Published In

Science

DOI

EISSN

1095-9203

Publication Date

December 19, 2008

Volume

322

Issue

5909

Start / End Page

1855 / 1857

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • RNA, Messenger
  • RNA, Antisense
  • Promoter Regions, Genetic
  • Leukocytes, Mononuclear
  • Introns
  • Humans
  • Genome, Human
  • General Science & Technology
  • Gene Expression Profiling
 

Citation

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He, Y., Vogelstein, B., Velculescu, V. E., Papadopoulos, N., & Kinzler, K. W. (2008). The antisense transcriptomes of human cells. Science, 322(5909), 1855–1857. https://doi.org/10.1126/science.1163853
He, Yiping, Bert Vogelstein, Victor E. Velculescu, Nickolas Papadopoulos, and Kenneth W. Kinzler. “The antisense transcriptomes of human cells.Science 322, no. 5909 (December 19, 2008): 1855–57. https://doi.org/10.1126/science.1163853.
He Y, Vogelstein B, Velculescu VE, Papadopoulos N, Kinzler KW. The antisense transcriptomes of human cells. Science. 2008 Dec 19;322(5909):1855–7.
He, Yiping, et al. “The antisense transcriptomes of human cells.Science, vol. 322, no. 5909, Dec. 2008, pp. 1855–57. Pubmed, doi:10.1126/science.1163853.
He Y, Vogelstein B, Velculescu VE, Papadopoulos N, Kinzler KW. The antisense transcriptomes of human cells. Science. 2008 Dec 19;322(5909):1855–1857.
Journal cover image

Published In

Science

DOI

EISSN

1095-9203

Publication Date

December 19, 2008

Volume

322

Issue

5909

Start / End Page

1855 / 1857

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • RNA, Messenger
  • RNA, Antisense
  • Promoter Regions, Genetic
  • Leukocytes, Mononuclear
  • Introns
  • Humans
  • Genome, Human
  • General Science & Technology
  • Gene Expression Profiling