Elucidating the Candida albicans calcineurin signaling cascade controlling stress response and virulence.

Journal Article (Journal Article)

The protein phosphatase calcineurin is a key mediator of virulence and antifungal susceptibility of multiple fungal pathogens including Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus, and has clinical potential as a therapeutic target to increase the efficacy of the current antifungal armamentarium. Despite the importance of this signaling pathway, few components of the calcineurin-signaling pathway are known in C. albicans. Here we identified and analyzed additional components of the C. albicans calcineurin cascade, including the RCN1 (regulator of calcineurin1), MID1, and CCH1 genes, which mediate calcineurin functions in other species. When heterologously expressed in Saccharomyces cerevisiae, C. albicans Rcn1 inhibited calcineurin function. Although rcn1/rcn1, mid1/mid1, and cch1/cch1 mutant strains share some phenotypes with calcineurin mutants, they do not completely recapitulate the phenotypes of a calcineurin mutant strain. These studies extend our understanding of the C. albicans calcineurin signaling cascade and its host-niche specific role in virulence.

Full Text

Duke Authors

Cited Authors

  • Reedy, JL; Filler, SG; Heitman, J

Published Date

  • February 2010

Published In

Volume / Issue

  • 47 / 2

Start / End Page

  • 107 - 116

PubMed ID

  • 19755168

Pubmed Central ID

  • PMC2815136

Electronic International Standard Serial Number (EISSN)

  • 1096-0937

Digital Object Identifier (DOI)

  • 10.1016/j.fgb.2009.09.002


  • eng

Conference Location

  • United States