Protein fabrication automation.

Published

Journal Article

Facile "writing" of DNA fragments that encode entire gene sequences potentially has widespread applications in biological analysis and engineering. Rapid writing of open reading frames (ORFs) for expressed proteins could transform protein engineering and production for protein design, synthetic biology, and structural analysis. Here we present a process, protein fabrication automation (PFA), which facilitates the rapid de novo construction of any desired ORF from oligonucleotides with low effort, high speed, and little human interaction. PFA comprises software for sequence design, data management, and the generation of instruction sets for liquid-handling robotics, a liquid-handling robot, a robust PCR scheme for gene assembly from synthetic oligonucleotides, and a genetic selection system to enrich correctly assembled full-length synthetic ORFs. The process is robust and scalable.

Full Text

Duke Authors

Cited Authors

  • Cox, JC; Lape, J; Sayed, MA; Hellinga, HW

Published Date

  • March 2007

Published In

Volume / Issue

  • 16 / 3

Start / End Page

  • 379 - 390

PubMed ID

  • 17242375

Pubmed Central ID

  • 17242375

Electronic International Standard Serial Number (EISSN)

  • 1469-896X

International Standard Serial Number (ISSN)

  • 0961-8368

Digital Object Identifier (DOI)

  • 10.1110/ps.062591607

Language

  • eng