Periplasmic binding proteins: a versatile superfamily for protein engineering.

Published

Journal Article (Review)

The diversity of biological function, ligand binding, conformational changes and structural adaptability of the periplasmic binding protein superfamily have been exploited to engineer biosensors, allosteric control elements, biologically active receptors and enzymes using a combination of techniques, including computational design. Extensively redesigned periplasmic binding proteins have been re-introduced into bacteria to function in synthetic signal transduction pathways that respond to extracellular ligands and as biologically active enzymes.

Full Text

Duke Authors

Cited Authors

  • Dwyer, MA; Hellinga, HW

Published Date

  • August 2004

Published In

Volume / Issue

  • 14 / 4

Start / End Page

  • 495 - 504

PubMed ID

  • 15313245

Pubmed Central ID

  • 15313245

International Standard Serial Number (ISSN)

  • 0959-440X

Digital Object Identifier (DOI)

  • 10.1016/j.sbi.2004.07.004

Language

  • eng

Conference Location

  • England