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Bacterial colonization of amniotic fluid in the presence of ruptured membranes.

Publication ,  Journal Article
Miller, JM; Hill, GB; Welt, SI; Pupkin, MJ
Published in: Am J Obstet Gynecol
June 15, 1980

Amniotic fluid (AF) was collected from 37 selected patients by amniocentesis, aspiration through a pressure catheter, or aspiration at the time of cesarean section. The unspun AF was examined directly by Gram stain for bacteria and white blood cells (WBC) and was cultured. Thirteen AF cultures were positive, defined as growth on primary plating media which corresponded to greater than 10(2) colony-forming units (CFU) per milliliter. Almost equal numbers of aerobic and anaerobic bacteria were isolated. The presence of bacteria, but not WBC, on Gram stain of AF correlated significantly with a positive culture, which indicated that microscopic examination of AF would usually predict the culture result. Growth of greater than 10(2) CFU/ml from AF was significantly associated with clinical chorioamnionitis, but colonization also was observed in five afebrile patients, four of whom were in premature labor. In patients delivered by cesarean section, bacteria on Gram stain and a positive culture from AF each were significantly correlated with postpartum endometritis.

Duke Scholars

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

June 15, 1980

Volume

137

Issue

4

Start / End Page

451 / 458

Location

United States

Related Subject Headings

  • Vagina
  • Time Factors
  • Risk
  • Pregnancy
  • Obstetrics & Reproductive Medicine
  • Labor, Obstetric
  • Inflammation
  • Infant, Newborn
  • Humans
  • Gestational Age
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Miller, J. M., Hill, G. B., Welt, S. I., & Pupkin, M. J. (1980). Bacterial colonization of amniotic fluid in the presence of ruptured membranes. Am J Obstet Gynecol, 137(4), 451–458. https://doi.org/10.1016/0002-9378(80)91126-6
Miller, J. M., G. B. Hill, S. I. Welt, and M. J. Pupkin. “Bacterial colonization of amniotic fluid in the presence of ruptured membranes.Am J Obstet Gynecol 137, no. 4 (June 15, 1980): 451–58. https://doi.org/10.1016/0002-9378(80)91126-6.
Miller JM, Hill GB, Welt SI, Pupkin MJ. Bacterial colonization of amniotic fluid in the presence of ruptured membranes. Am J Obstet Gynecol. 1980 Jun 15;137(4):451–8.
Miller, J. M., et al. “Bacterial colonization of amniotic fluid in the presence of ruptured membranes.Am J Obstet Gynecol, vol. 137, no. 4, June 1980, pp. 451–58. Pubmed, doi:10.1016/0002-9378(80)91126-6.
Miller JM, Hill GB, Welt SI, Pupkin MJ. Bacterial colonization of amniotic fluid in the presence of ruptured membranes. Am J Obstet Gynecol. 1980 Jun 15;137(4):451–458.
Journal cover image

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

June 15, 1980

Volume

137

Issue

4

Start / End Page

451 / 458

Location

United States

Related Subject Headings

  • Vagina
  • Time Factors
  • Risk
  • Pregnancy
  • Obstetrics & Reproductive Medicine
  • Labor, Obstetric
  • Inflammation
  • Infant, Newborn
  • Humans
  • Gestational Age