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Effects of handling on heat shock protein expression in rainbow trout (Oncorhynchus mykiss).

Publication ,  Journal Article
Washburn, BS; Moreland, JJ; Slaughter, AM; Werner, I; Hinton, DE; Sanders, BM
Published in: Environmental toxicology and chemistry
March 2002

As part of an effort to validate the use of heat shock proteins (HSPs) as biomarkers of exposure to and effects of contaminants, we evaluated the effect of two handling regimens on the induction of HSP 60 and 70 in rainbow trout (Oncorhynchus mykiss). Fish were acclimated to laboratory conditions for several weeks before the beginning of the experiment. Fish were then captured by net, placed in a cooler for 1 h while being transported in a truck, returned to their original tanks, then sacrificed 6 to 8 h later. Tricaine methane sulfonate (MS-222) was used during different phases of handling to reduce handling stress. Heat-stressed fish were included in the experiment as a positive control. Muscle, liver, gills, and heart were analyzed for HSP 60 and 70 by immunoblotting. We found no effect of any handling regimen on the induction of HSPs. These findings suggest that the capture and transport of fish for environmental monitoring purposes should not interfere with the use of stress proteins as biomarkers.

Duke Scholars

Published In

Environmental toxicology and chemistry

DOI

EISSN

1552-8618

ISSN

0730-7268

Publication Date

March 2002

Volume

21

Issue

3

Start / End Page

557 / 560

Related Subject Headings

  • Tissue Distribution
  • Specimen Handling
  • Reproducibility of Results
  • Oncorhynchus mykiss
  • Muscle, Skeletal
  • Liver
  • Immunoblotting
  • HSP70 Heat-Shock Proteins
  • Gills
  • Environmental Sciences
 

Citation

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Washburn, B. S., Moreland, J. J., Slaughter, A. M., Werner, I., Hinton, D. E., & Sanders, B. M. (2002). Effects of handling on heat shock protein expression in rainbow trout (Oncorhynchus mykiss). Environmental Toxicology and Chemistry, 21(3), 557–560. https://doi.org/10.1002/etc.5620210313
Washburn, Barbara Shayne, Jason J. Moreland, Ann M. Slaughter, Inge Werner, David E. Hinton, and Brenda M. Sanders. “Effects of handling on heat shock protein expression in rainbow trout (Oncorhynchus mykiss).Environmental Toxicology and Chemistry 21, no. 3 (March 2002): 557–60. https://doi.org/10.1002/etc.5620210313.
Washburn BS, Moreland JJ, Slaughter AM, Werner I, Hinton DE, Sanders BM. Effects of handling on heat shock protein expression in rainbow trout (Oncorhynchus mykiss). Environmental toxicology and chemistry. 2002 Mar;21(3):557–60.
Washburn, Barbara Shayne, et al. “Effects of handling on heat shock protein expression in rainbow trout (Oncorhynchus mykiss).Environmental Toxicology and Chemistry, vol. 21, no. 3, Mar. 2002, pp. 557–60. Epmc, doi:10.1002/etc.5620210313.
Washburn BS, Moreland JJ, Slaughter AM, Werner I, Hinton DE, Sanders BM. Effects of handling on heat shock protein expression in rainbow trout (Oncorhynchus mykiss). Environmental toxicology and chemistry. 2002 Mar;21(3):557–560.
Journal cover image

Published In

Environmental toxicology and chemistry

DOI

EISSN

1552-8618

ISSN

0730-7268

Publication Date

March 2002

Volume

21

Issue

3

Start / End Page

557 / 560

Related Subject Headings

  • Tissue Distribution
  • Specimen Handling
  • Reproducibility of Results
  • Oncorhynchus mykiss
  • Muscle, Skeletal
  • Liver
  • Immunoblotting
  • HSP70 Heat-Shock Proteins
  • Gills
  • Environmental Sciences