Skip to main content
Journal cover image

Spatial profiles of hsp70 proteins in Asian clam (Potamocorbula amurensis) in northern San Francisco Bay may be linked to natural rather than anthropogenic stressors.

Publication ,  Journal Article
Werner, I; Hinton, DE
Published in: Marine environmental research
July 2000

Multi-year investigations in northern San Francisco Bay by United States Geological Survey have linked reduced condition indices in populations of Asian clam (Potamocorbula amurensis) with elevated cadmium tissue concentrations. Our study seeks to determine whether levels of hsp70 proteins in P. amurensis can be correlated with these findings, and/or are related to histopathologic alterations and concentrations of metallothionein-like proteins. Here we present our results on stress proteins in clams collected monthly from four field stations between July 1996 and January 1998. In addition, animals were exposed in the laboratory to a range of salinities. Stress proteins were analyzed by Western blotting using monoclonal antibodies. Hsp70 protein levels in field-collected clams were significantly higher at the seaward (high salinity/low cadmium) stations (12.5, 8.1) than at the landward (low salinity/high cadmium) stations (6.1, 4.1). Laboratory studies showed that clams exposed to 0.1 ppt salinity had markedly lower hsp70 levels than clams exposed to higher salinities. In view of our previous laboratory studies showing that cadmium induces hsp70 in P. amurensis, our present results indicate that reduced hsp70 protein levels in field-collected clams may be linked to salinity effects rather than cadmium tissue concentrations.

Duke Scholars

Published In

Marine environmental research

DOI

EISSN

1879-0291

ISSN

0141-1136

Publication Date

July 2000

Volume

50

Issue

1-5

Start / End Page

379 / 384

Related Subject Headings

  • San Francisco
  • Marine Biology & Hydrobiology
  • HSP70 Heat-Shock Proteins
  • Geology
  • Geological Phenomena
  • Cadmium
  • Blotting, Western
  • Bivalvia
  • Antibodies, Monoclonal
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Werner, I., & Hinton, D. E. (2000). Spatial profiles of hsp70 proteins in Asian clam (Potamocorbula amurensis) in northern San Francisco Bay may be linked to natural rather than anthropogenic stressors. Marine Environmental Research, 50(1–5), 379–384. https://doi.org/10.1016/s0141-1136(00)00058-1
Werner, I., and D. E. Hinton. “Spatial profiles of hsp70 proteins in Asian clam (Potamocorbula amurensis) in northern San Francisco Bay may be linked to natural rather than anthropogenic stressors.Marine Environmental Research 50, no. 1–5 (July 2000): 379–84. https://doi.org/10.1016/s0141-1136(00)00058-1.
Werner, I., and D. E. Hinton. “Spatial profiles of hsp70 proteins in Asian clam (Potamocorbula amurensis) in northern San Francisco Bay may be linked to natural rather than anthropogenic stressors.Marine Environmental Research, vol. 50, no. 1–5, July 2000, pp. 379–84. Epmc, doi:10.1016/s0141-1136(00)00058-1.
Journal cover image

Published In

Marine environmental research

DOI

EISSN

1879-0291

ISSN

0141-1136

Publication Date

July 2000

Volume

50

Issue

1-5

Start / End Page

379 / 384

Related Subject Headings

  • San Francisco
  • Marine Biology & Hydrobiology
  • HSP70 Heat-Shock Proteins
  • Geology
  • Geological Phenomena
  • Cadmium
  • Blotting, Western
  • Bivalvia
  • Antibodies, Monoclonal
  • Animals