Fish-based biomonitoring to determine toxic characteristics of complex chemical mixtures: documentation of bioremediation at a pesticide disposal site
Medaka (Oryzias latipes) embryo and larval short-term bioassays were used to rapidly demonstrate changes in toxicity of aqueous soil extracts at a pesticide disposal site before and after bioremediation. By chemical analysis, concentrations of most pesticides (particularly endosulfans) decreased after the 63-day bioremediation. In static larval assays of extracts before bioremediation, concentrations from 2.2 to 2.9% produced 50% larval mortality after 72 h exposure, whereas extract concentrations of 18 to 34% were required after remediation. Extract concentrations required to inhibit normal embryo development increased from 10% before bioremediation to 20% afterwards. Aqueous extracts from untreated soil were nonmutagenic (Ames bioassay) and, nine months after a 72-h exposure, were associated with no histopath-ologic alterations. Advantages inherent with medaka embryo and larval bioassay indicate that fish-based biomonitoring might significantly improve efforts to characterize hazardous waste sites. © 1991.
Duke Scholars
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Related Subject Headings
- Toxicology
- 06 Biological Sciences
- 05 Environmental Sciences
- 03 Chemical Sciences
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- Toxicology
- 06 Biological Sciences
- 05 Environmental Sciences
- 03 Chemical Sciences