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Induction of differentiation in human myeloblastic leukemia ML-1 cells by heptachlor, a chlorinated hydrocarbon insecticide.

Publication ,  Journal Article
Chuang, LF; Hinton, DE; Cheung, AT; Chuang, RY
Published in: Toxicology and applied pharmacology
June 1991

Effects of heptachlor, an organochlorine pesticide, on human myeloblastic leukemia ML-1 cells were determined. Similar to 12-O-tetradecanoylphorbol-13-acetate (TPA), a known tumor promoter, heptachlor induced cell adherence and formation of extended cytoplasmic pseudopodia in ML-1 cells. The growth of ML-1 was slightly stimulated by low concentrations (less than 30 nM) of heptachlor. A dose-responsive cell death was also observed when ML-1 cells were treated with heptachlor at concentrations greater than 80 microM. Examination by light microscopy of the cells treated with 80 microM heptachlor revealed a gradual appearance of differentiation characteristics in the culture. On Day 3 of the treatment, 41% of the cells remained unchanged as ML-1, 39% of the cells showed changes and apparent cell differentiation, and 20% of the cells were induced to differentiate to monocyte- or macrophage-like cell type. Electron microscopy also revealed cellular differentiation and the presence of monocyte- and macrophage-like cell types (22%) was confirmed by positive esterase staining.

Duke Scholars

Published In

Toxicology and applied pharmacology

DOI

EISSN

1096-0333

ISSN

0041-008X

Publication Date

June 1991

Volume

109

Issue

1

Start / End Page

98 / 107

Related Subject Headings

  • Tumor Cells, Cultured
  • Toxicology
  • Naphthol AS D Esterase
  • Microscopy
  • Leukemia, Myeloid
  • Humans
  • Heptachlor
  • Cell Survival
  • Cell Division
  • Cell Differentiation
 

Citation

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Chuang, L. F., Hinton, D. E., Cheung, A. T., & Chuang, R. Y. (1991). Induction of differentiation in human myeloblastic leukemia ML-1 cells by heptachlor, a chlorinated hydrocarbon insecticide. Toxicology and Applied Pharmacology, 109(1), 98–107. https://doi.org/10.1016/0041-008x(91)90194-j
Chuang, L. F., D. E. Hinton, A. T. Cheung, and R. Y. Chuang. “Induction of differentiation in human myeloblastic leukemia ML-1 cells by heptachlor, a chlorinated hydrocarbon insecticide.Toxicology and Applied Pharmacology 109, no. 1 (June 1991): 98–107. https://doi.org/10.1016/0041-008x(91)90194-j.
Chuang LF, Hinton DE, Cheung AT, Chuang RY. Induction of differentiation in human myeloblastic leukemia ML-1 cells by heptachlor, a chlorinated hydrocarbon insecticide. Toxicology and applied pharmacology. 1991 Jun;109(1):98–107.
Chuang, L. F., et al. “Induction of differentiation in human myeloblastic leukemia ML-1 cells by heptachlor, a chlorinated hydrocarbon insecticide.Toxicology and Applied Pharmacology, vol. 109, no. 1, June 1991, pp. 98–107. Epmc, doi:10.1016/0041-008x(91)90194-j.
Chuang LF, Hinton DE, Cheung AT, Chuang RY. Induction of differentiation in human myeloblastic leukemia ML-1 cells by heptachlor, a chlorinated hydrocarbon insecticide. Toxicology and applied pharmacology. 1991 Jun;109(1):98–107.
Journal cover image

Published In

Toxicology and applied pharmacology

DOI

EISSN

1096-0333

ISSN

0041-008X

Publication Date

June 1991

Volume

109

Issue

1

Start / End Page

98 / 107

Related Subject Headings

  • Tumor Cells, Cultured
  • Toxicology
  • Naphthol AS D Esterase
  • Microscopy
  • Leukemia, Myeloid
  • Humans
  • Heptachlor
  • Cell Survival
  • Cell Division
  • Cell Differentiation