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Morphological changes in trout hepatocytes exposed to acetaminophen

Publication ,  Journal Article
Blair, JB; Hinton, DE; Miller, MR
Published in: Marine Environmental Research
January 1, 1989

Isolated trout hepatocytes prepared by collagenase digestion were maintained in primary culture in chemically defined, serum-free medium for 3-5 days to evaluate the influence of various agents on cell-cell interactions. Isolated cells were plated onto plastic culture plates coated with trout skin extracts. Greater than 80% of the lactate dehydrogenase activity (LDH) and DNA content of the cells was retained for 3 days, indicating good cell viability. After 24 h, the cells aggregate into clumps which increase in size over several days to include several hundred cells, four to ten cells deep on the surface of the plate. Microscopic evaluation suggests these aggregates reflect normal cell-cell interactions. Adjacent hepatocytes appear to share junctional complexes and form canaliculi. Cells ultrastructurally identical to biliary epithelial cells form apparent ductule complexes. Acetaminophen (2-10mm) interferes with cell-cell aggregation. Treated hepatocytes remain rounded and mitochondria distribution is altered, occupying only the central region of the cells. Acetaminophen has little influence on LDH or DNA content, suggesting a sensitive and specific action of the drug on cell-cell interactions. © 1990.

Duke Scholars

Published In

Marine Environmental Research

DOI

ISSN

0141-1136

Publication Date

January 1, 1989

Volume

28

Issue

1-4

Start / End Page

357 / 361

Related Subject Headings

  • Marine Biology & Hydrobiology
  • 06 Biological Sciences
  • 05 Environmental Sciences
  • 03 Chemical Sciences
 

Citation

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Blair, J. B., Hinton, D. E., & Miller, M. R. (1989). Morphological changes in trout hepatocytes exposed to acetaminophen. Marine Environmental Research, 28(1–4), 357–361. https://doi.org/10.1016/0141-1136(89)90262-6
Blair, J. B., D. E. Hinton, and M. R. Miller. “Morphological changes in trout hepatocytes exposed to acetaminophen.” Marine Environmental Research 28, no. 1–4 (January 1, 1989): 357–61. https://doi.org/10.1016/0141-1136(89)90262-6.
Blair JB, Hinton DE, Miller MR. Morphological changes in trout hepatocytes exposed to acetaminophen. Marine Environmental Research. 1989 Jan 1;28(1–4):357–61.
Blair, J. B., et al. “Morphological changes in trout hepatocytes exposed to acetaminophen.” Marine Environmental Research, vol. 28, no. 1–4, Jan. 1989, pp. 357–61. Scopus, doi:10.1016/0141-1136(89)90262-6.
Blair JB, Hinton DE, Miller MR. Morphological changes in trout hepatocytes exposed to acetaminophen. Marine Environmental Research. 1989 Jan 1;28(1–4):357–361.
Journal cover image

Published In

Marine Environmental Research

DOI

ISSN

0141-1136

Publication Date

January 1, 1989

Volume

28

Issue

1-4

Start / End Page

357 / 361

Related Subject Headings

  • Marine Biology & Hydrobiology
  • 06 Biological Sciences
  • 05 Environmental Sciences
  • 03 Chemical Sciences