Skip to main content
Journal cover image

Mechanism of allyl formate-induced hepatotoxicity in rainbow trout.

Publication ,  Journal Article
Droy, BF; Davis, ME; Hinton, DE
Published in: Toxicology and applied pharmacology
April 1989

Hepatotoxicity of allyl formate (AF) was studied in trout, to characterize the response of the teleost liver to a mammalian periportal hepatotoxicant. A dose-dependent decrease in liver nonprotein sulfhydryl (NPSH) concentration was observed at 3, 6, and 24 hr following 9.5, 28, and 95 mg/kg) AF with maximal depression seen at 6 hr (51, 40, and 29% control, respectively). Further evidence for glutathione (GSH) protection against AF toxicity was seen when diethylmaleate, a GSH depleting agent (0.6 ml/kg ip), administered 30 min prior to AF (9.5 and 28 mg/kg), increased AF hepatotoxicity (10-fold shift in the dose-response effect on SGPT). Also, N-acetyl-L-cysteine (150 mg/kg ip), a GSH precursor, protected liver against AF toxicity when injected 5 min prior to and 1, 5, and 9 hr after AF (28 and 95 mg/kg). Pyrazole (375 mg/kg ip), an alcohol dehydrogenase inhibitor, given 4 hr before AF (95 mg/kg), attenuated the histopathological effect of AF. These results indicate that AF, once bioactivated by alcohol dehydrogenase, causes significant toxicity in trout liver. GSH protects against AF-induced effects since greater than 50% decreases in liver GSH are required before toxicity is expressed.

Duke Scholars

Published In

Toxicology and applied pharmacology

DOI

EISSN

1096-0333

ISSN

0041-008X

Publication Date

April 1989

Volume

98

Issue

2

Start / End Page

313 / 324

Related Subject Headings

  • Trout
  • Toxicology
  • Sulfhydryl Compounds
  • Maleates
  • Liver
  • Glutathione
  • Formic Acid Esters
  • Formates
  • Dose-Response Relationship, Drug
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Droy, B. F., Davis, M. E., & Hinton, D. E. (1989). Mechanism of allyl formate-induced hepatotoxicity in rainbow trout. Toxicology and Applied Pharmacology, 98(2), 313–324. https://doi.org/10.1016/0041-008x(89)90236-6
Droy, B. F., M. E. Davis, and D. E. Hinton. “Mechanism of allyl formate-induced hepatotoxicity in rainbow trout.Toxicology and Applied Pharmacology 98, no. 2 (April 1989): 313–24. https://doi.org/10.1016/0041-008x(89)90236-6.
Droy BF, Davis ME, Hinton DE. Mechanism of allyl formate-induced hepatotoxicity in rainbow trout. Toxicology and applied pharmacology. 1989 Apr;98(2):313–24.
Droy, B. F., et al. “Mechanism of allyl formate-induced hepatotoxicity in rainbow trout.Toxicology and Applied Pharmacology, vol. 98, no. 2, Apr. 1989, pp. 313–24. Epmc, doi:10.1016/0041-008x(89)90236-6.
Droy BF, Davis ME, Hinton DE. Mechanism of allyl formate-induced hepatotoxicity in rainbow trout. Toxicology and applied pharmacology. 1989 Apr;98(2):313–324.
Journal cover image

Published In

Toxicology and applied pharmacology

DOI

EISSN

1096-0333

ISSN

0041-008X

Publication Date

April 1989

Volume

98

Issue

2

Start / End Page

313 / 324

Related Subject Headings

  • Trout
  • Toxicology
  • Sulfhydryl Compounds
  • Maleates
  • Liver
  • Glutathione
  • Formic Acid Esters
  • Formates
  • Dose-Response Relationship, Drug
  • Animals