Whole-organism screening for gluconeogenesis identifies activators of fasting metabolism.

Journal Article (Journal Article)

Improving the control of energy homeostasis can lower cardiovascular risk in metabolically compromised individuals. To identify new regulators of whole-body energy control, we conducted a high-throughput screen in transgenic reporter zebrafish for small molecules that modulate the expression of the fasting-inducible gluconeogenic gene pck1. We show that this in vivo strategy identified several drugs that affect gluconeogenesis in humans as well as metabolically uncharacterized compounds. Most notably, we find that the translocator protein ligands PK 11195 and Ro5-4864 are glucose-lowering agents despite a strong inductive effect on pck1 expression. We show that these drugs are activators of a fasting-like energy state and, notably, that they protect high-fat diet-induced obese mice from hepatosteatosis and glucose intolerance, two pathological manifestations of metabolic dysregulation. Thus, using a whole-organism screening strategy, this study has identified new small-molecule activators of fasting metabolism.

Full Text

Duke Authors

Cited Authors

  • Gut, P; Baeza-Raja, B; Andersson, O; Hasenkamp, L; Hsiao, J; Hesselson, D; Akassoglou, K; Verdin, E; Hirschey, MD; Stainier, DYR

Published Date

  • February 2013

Published In

Volume / Issue

  • 9 / 2

Start / End Page

  • 97 - 104

PubMed ID

  • 23201900

Pubmed Central ID

  • PMC3552031

Electronic International Standard Serial Number (EISSN)

  • 1552-4469

Digital Object Identifier (DOI)

  • 10.1038/nchembio.1136


  • eng

Conference Location

  • United States