Lung development and repair: contribution of the ciliated lineage.

Published

Journal Article

The identity of the endogenous epithelial cells in the adult lung that are responsible for normal turnover and repair after injury is still controversial. In part, this is due to a paucity of highly specific genetic lineage tools to follow efficiently the fate of the major epithelial cell populations: the basal, secretory, ciliated, neuroendocrine, and alveolar cells. As part of a program to address this problem we have used a 1-kb FOXJ1 promoter to drive CreER in the ciliated cells of the embryonic and adult lung. Analysis of FOXJ1-GFP transgenic lungs shows that labeled cells appear in a proximal-distal pattern during embryogenesis and that the promoter drives expression in all ciliated cells. Using FOXJ1CreER adult mice, we have followed the fate of ciliated cells after epithelial injury by naphthalene or sulfur dioxide. From quantitative analysis and confocal microscopy we conclude that ciliated cells transiently change their morphology in response to lung injury but do not proliferate or transdifferentiate as part of the repair process.

Full Text

Duke Authors

Cited Authors

  • Rawlins, EL; Ostrowski, LE; Randell, SH; Hogan, BLM

Published Date

  • January 9, 2007

Published In

Volume / Issue

  • 104 / 2

Start / End Page

  • 410 - 417

PubMed ID

  • 17194755

Pubmed Central ID

  • 17194755

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0610770104

Language

  • eng

Conference Location

  • United States