Gene targeting in mice reveals a requirement for angiotensin in the development and maintenance of kidney morphology and growth factor regulation.

Published

Journal Article

Elevated levels of endogenous angiotensin can cause hypertensive nephrosclerosis as a result of the potent vasopressor action of the peptide. We have produced by gene targeting mice homozygous for a null mutation in the angiotensinogen gene (Atg-1-). Postnatally, Atg-1- animals show a modest delay in glomerular maturation. Although Atg-1- animals are hypotensive by 7 wk of age, they develop, by 3 wk of age, pronounced lesions in the renal cortex, similar to those of hypertensive nephrosclerosis. In addition, the papillae of homozygous mutant kidneys are reduced in size. These lesions are accompanied by local up-regulation of PDGF-B and TGF-beta1 mRNA in the cortex and down-regulation of PDGF-A mRNA in the papilla. The study demonstrates an important requirement for angiotensin in achieving and maintaining the normal morphology of the kidney. The mechanism through which angiotensin maintains the volume homeostasis in mammals includes promotion of the maturational growth of the papilla.

Full Text

Duke Authors

Cited Authors

  • Niimura, F; Labosky, PA; Kakuchi, J; Okubo, S; Yoshida, H; Oikawa, T; Ichiki, T; Naftilan, AJ; Fogo, A; Inagami, T

Published Date

  • December 1995

Published In

Volume / Issue

  • 96 / 6

Start / End Page

  • 2947 - 2954

PubMed ID

  • 8675666

Pubmed Central ID

  • 8675666

International Standard Serial Number (ISSN)

  • 0021-9738

Digital Object Identifier (DOI)

  • 10.1172/JCI118366

Language

  • eng

Conference Location

  • United States