Drosophila topoisomerase II double-strand DNA cleavage: analysis of DNA sequence homology at the cleavage site.


Journal Article

In order to study the sequence specificity of double-strand DNA cleavage by Drosophila topoisomerase II, we have mapped and sequenced 16 strong and 47 weak cleavage sites in the recombinant plasmid p pi 25.1. Analysis of the nucleotide and dinucleotide frequencies in the region near the site of phosphodiester bond breakage revealed a nonrandom distribution. The nucleotide frequencies observed would occur by chance with a probability less than 0.05. The consensus sequence we derived is 5'GT.A/TAY decrease ATT.AT..G 3', where a dot means no preferred nucleotide, Y is for pyrimidine, and the arrow shows the point of bond cleavage. On average, strong sites match the consensus better than weak sites.

Full Text

Cited Authors

  • Sander, M; Hsieh, TS

Published Date

  • February 25, 1985

Published In

Volume / Issue

  • 13 / 4

Start / End Page

  • 1057 - 1072

PubMed ID

  • 2987816

Pubmed Central ID

  • 2987816

International Standard Serial Number (ISSN)

  • 0305-1048

Digital Object Identifier (DOI)

  • 10.1093/nar/13.4.1057


  • eng

Conference Location

  • England